ArticleApplied and environmental microbiology2008
Interactions of Cryptosporidium parvum, Giardia lamblia, vaccinal poliovirus type 1, and bacteriophages phiX174 and MS2 with a drinking water biofilm and a wastewater biofilm.
Article in Applied and environmental microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
28 citing papers in PubMed, 1 synthesis or guideline pooled it.
- How long do pathogens persist and survive in water? A systematic review.Frontiers in microbiology · 2025Pooled it
- Micropollutants and Their Interactions With Relevant Environmental Viruses.Environmental microbiology · 2025Review
- Biofilm detachment significantly affects biological stability of drinking water during intermittent water supply in a pilot scale water distribution system.Scientific reports · 2025Article
- Cross-Cutting Approach for the Characterization of Microbial Emerging Hazards in Agriculture Settings from Circular Economy-Driven Wastewater Streams.Environment & health (Washington, D.C.) · 2024Review
- The Association ofInternational journal of molecular sciences · 2023Article
- Interactions Between Infectious Foodborne Viruses and Bacterial Biofilms Formed on Different Food Contact Surfaces.Food and environmental virology · 2022Article
- Regulatory network controls microbial biofilm development, withBioengineered · 2022Review
- Biofilms and Coronavirus Reservoirs: a Perspective Review.Applied and environmental microbiology · 2021Review
- Review
- Biofilm Sampling for Detection ofApplied and environmental microbiology · 2020Article
- Two Drinking Water Outbreaks Caused by Wastewater Intrusion Including Sapovirus in Finland.International journal of environmental research and public health · 2019Article
- Influence of physico-chemical characteristics of sediment on the in situ spatial distribution of F-specific RNA phages in the riverbed.FEMS microbiology ecology · 2019Article
- The use of wastewater in livestock production and its socioeconomic and welfare implications.Environmental science and pollution research international · 2017Review
- Pathogen and Particle Associations in Wastewater: Significance and Implications for Treatment and Disinfection Processes.Advances in applied microbiology · 2016Review
- Human and animal enteric virus in groundwater from deep wells, and recreational and network water.Environmental science and pollution research international · 2015Article
- Diversity and functions of bacterial community in drinking water biofilms revealed by high-throughput sequencing.Scientific reports · 2015Article
- Microbial removals by a novel biofilter water treatment system.The American journal of tropical medicine and hygiene · 2015Article
- Health risk assessment related to waterborne pathogens from the river to the tap.International journal of environmental research and public health · 2015Article
- Recombinant adenovirus as a model to evaluate the efficiency of free chlorine disinfection in filtered water samples.Virology journal · 2015Article
- Impact of Bioreactor Environment and Recovery Method on the Profile of Bacterial Populations from Water Distribution Systems.PloS one · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Biofilms colonizing surfaces inside drinking water distribution networks may provide a habitat and shelter to pathogenic viruses and parasites. If released from biofilms, these pathogens may disseminate in the water distribution system and cause waterborne diseases. Our study aimed to investigate the interactions of protozoan parasites (Cryptosporidium parvum and Giardia lamblia [oo]cysts) and viruses (vaccinal poliovirus type 1, phiX174, and MS2) with two contrasting biofilms. First, attachment, persistence, and detachment of the protozoan parasites and the viruses were assessed with a drinking water biofilm. This biofilm was allowed to develop inside a rotating annular reactor fed with tap water for 7 months prior to the inoculation. Our results show that viable parasites and infectious viruses attached to the drinking water biofilm within 1 h and persisted within the biofilm. Indeed, infectious viruses were detected in the drinking water biofilm up to 6 days after the inoculation, while viral genome and viable parasites were still detected at day 34, corresponding to the last day of the monitoring period. Since viral genome was detected much longer than infectious particles, our results raise the question of the significance of detecting viral genomes in biofilms. A transfer of viable parasites and viruses from the biofilm to the water phase was observed after the flow velocity was increased but also with a constant laminar flow rate. Similar results regarding parasite and virus attachment and detachment were obtained using a treated wastewater biofilm, suggesting that our observations might be extrapolated to a wide range of environmental biofilms and confirming that biofilms can be considered a potential secondary source of contamination.
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Registered trials
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