ArticleThe Journal of experimental biology2008
Insulin regulates aging and oxidative stress in Anopheles stephensi.
Article in The Journal of experimental biology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed, 67 citations in OpenAlex.
- Blood feeding, microbiota, and layered mosquito immunity: from pathway phenotypes to vector competence.Frontiers in immunology · 2026Review
- Genomic and insulin-mediated control of metabolic homeostasis by the mosquito ecdysone-induced gene E93.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- A diet-induced obese and diabetic host phenotype reduces mosquito ZIKV infections and remodels gut metabolism.Frontiers in immunology · 2025Article
- Evolution of innate immunity: lessons from mammalian models shaping our current view of insect immunity.Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology · 2024Review
- Article
- Western diet consumption by host vertebrate promotes altered gene expression on Aedes aegypti reducing its lifespan and increasing fertility following blood feeding.Parasites & vectors · 2024Article
- Sustained antiviral insulin signaling during West Nile virus infection results in viral mutations.Frontiers in cellular and infection microbiology · 2024Article
- Expansive and Diverse Phenotypic Landscape of Field Aedes aegypti (Diptera: Culicidae) Larvae with Differential Susceptibility to Temephos: Beyond Metabolic Detoxification.Journal of medical entomology · 2022Article
- Sugar feeding protects against arboviral infection by enhancing gut immunity in the mosquito vector Aedes aegypti.PLoS pathogens · 2021Article
- Vector Specificity of Arbovirus Transmission.Frontiers in microbiology · 2021Review
- Using Diverse Model Systems to Define Intestinal Epithelial Defenses to Enteric Viral Infections.Cell host & microbe · 2020Review
- The contribution of gut bacteria to insecticide resistance and the life histories of the major malaria vector Anopheles arabiensis (Diptera: Culicidae).Scientific reports · 2019Article
- Insulin-Like Peptide Signaling in Mosquitoes: The Road Behind and the Road Ahead.Frontiers in endocrinology · 2019Review
- Structures of insect Imp-L2 suggest an alternative strategy for regulating the bioavailability of insulin-like hormones.Nature communications · 2018Article
- Abscisic acid induces a transient shift in signaling that enhances NF-κB-mediated parasite killing in the midgut of Anopheles stephensi without reducing lifespan or fecundity.Parasites & vectors · 2017Article
- Article
- Two insulin-like peptides differentially regulate malaria parasite infection in the mosquito through effects on intermediary metabolism.The Biochemical journal · 2016Article
- Polyphenol-Rich Diets Exacerbate AMPK-Mediated Autophagy, Decreasing Proliferation of Mosquito Midgut Microbiota, and Extending Vector Lifespan.PLoS neglected tropical diseases · 2016Article
- Human C5a Protein Participates in the Mosquito Immune Response Against Dengue Virus.Journal of medical entomology · 2016Article
- Review
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Observations from nematodes to mammals indicate that insulin/insulin-like growth factor signaling (IIS) regulates lifespan. As in other organisms, IIS is conserved in mosquitoes and signaling occurs in multiple tissues. During bloodfeeding, mosquitoes ingest human insulin. This simple observation suggested that exogenous insulin could mimic the endogenous hormonal control of aging in mosquitoes, providing a new model to examine this phenomenon at the organismal and cellular levels. To this end, female Anopheles stephensi mosquitoes were maintained on diets containing human insulin provided daily in sucrose or three times weekly by artificial bloodmeal. Regardless of delivery route, mosquitoes provided with insulin at 1.7 x 10(-4) and 1.7 x 10(-3) micromol l(-1), doses 0.3-fold and 3.0-fold higher than non-fasting blood levels, died at a faster rate than controls. In mammals, IIS induces the synthesis of reactive oxygen species and downregulates antioxidants, events that increase oxidative stress and that have been associated with reduced lifespan. Insulin treatment of mosquito cells in vitro induced hydrogen peroxide synthesis while dietary supplementation reduced total superoxide dismutase (SOD) activity and manganese SOD activity relative to controls. The effects of insulin on mortality were reversed when diets were supplemented with manganese (III) tetrakis (4-benzoic acid) porphyrin (MnTBAP), a cell-permeable SOD mimetic agent, suggesting that insulin-induced mortality was due to oxidative stress. In addition, dietary insulin activated Akt/protein kinase B and extracellular signal-regulated kinase (ERK) in the mosquito midgut, suggesting that, as observed in Caenorhabditis elegans, the midgut may act as a 'signaling center' for mosquito aging.
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Registered trials
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