ArticleTranslational research : the journal of laboratory and clinical medicine2008
A transgenic mouse with vascular endothelial over-expression of the non-muscle myosin light chain kinase-2 isoform is susceptible to inflammatory lung injury: role of sexual dimorphism and age.
Article in Translational research : the journal of laboratory and clinical medicine, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
26 citing papers in PubMed, 1 synthesis or guideline pooled it, 37 citations in OpenAlex.
- A preclinical systematic review and meta-analysis assessing the effect of biological sex in lipopolysaccharide-induced acute lung injury.American journal of physiology. Lung cellular and molecular physiology · 2024Pooled it
- A Razor's Edge: Vascular Responses to Acute Inflammatory Lung Injury/Acute Respiratory Distress Syndrome.Annual review of physiology · 2024Review
- Critical role for the lung endothelial nonmuscle myosin light-chain kinase isoform in the severity of inflammatory murine lung injury.Pulmonary circulation · 2022Article
- The Calcium Signaling Mechanisms in Arterial Smooth Muscle and Endothelial Cells.Comprehensive Physiology · 2021Article
- Graft-versus-host disease propagation depends on increased intestinal epithelial tight junction permeability.The Journal of clinical investigation · 2019Article
- The Splicing Factor hnRNPA1 Regulates Alternate Splicing of the MYLK Gene.American journal of respiratory cell and molecular biology · 2018Article
- Splice Wars: The Role of MLCK Isoforms in Ventilation-induced Lung Injury.American journal of respiratory cell and molecular biology · 2018Article
- Dysregulated Nox4 ubiquitination contributes to redox imbalance and age-related severity of acute lung injury.American journal of physiology. Lung cellular and molecular physiology · 2017Article
- Mechanical Stress and Single Nucleotide Variants Regulate Alternative Splicing of the MYLK Gene.American journal of respiratory cell and molecular biology · 2017Article
- Sp1-mediated nonmuscle myosin light chain kinase expression and enhanced activity in vascular endothelial growth factor-induced vascular permeability.Pulmonary circulation · 2015Article
- Proline-rich region of non-muscle myosin light chain kinase modulates kinase activity and endothelial cytoskeletal dynamics.Microvascular research · 2014Article
- Nonmuscle myosin light chain kinase regulates murine asthmatic inflammation.American journal of respiratory cell and molecular biology · 2014Article
- Myosin light chain kinase signaling in endothelial barrier dysfunction.Medicinal research reviews · 2013Review
- Transcriptional events during the recovery from MRSA lung infection: a mouse pneumonia model.PloS one · 2013Article
- Linezolid Exerts Greater Bacterial Clearance but No Modification of Host Lung Gene Expression Profiling: A Mouse MRSA Pneumonia Model.PloS one · 2013Article
- Novel role for non-muscle myosin light chain kinase (MLCK) in hyperoxia-induced recruitment of cytoskeletal proteins, NADPH oxidase activation, and reactive oxygen species generation in lung endothelium.The Journal of biological chemistry · 2012Article
- A sphingosine 1-phosphate 1 receptor agonist modulates brain death-induced neurogenic pulmonary injury.American journal of respiratory cell and molecular biology · 2011Article
- Biochemistry of smooth muscle myosin light chain kinase.Archives of biochemistry and biophysics · 2011Review
- Simvastatin attenuates radiation-induced murine lung injury and dysregulated lung gene expression.American journal of respiratory cell and molecular biology · 2011Article
- Non-muscle myosin light chain kinase isoform is a viable molecular target in acute inflammatory lung injury.American journal of respiratory cell and molecular biology · 2011Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
We have generated genetically engineered mice that are uniquely susceptible to lipopolysaccharide (LPS)-induced and mechanical ventilation-induced lung injury in a sex-specific and age-specific manner. These mice express a nonmuscle isoform of the myosin light chain kinase gene (nmMLCK2) targeted to the endothelium. Homozygous mice have significantly reduced fecundity and litter survival until weaning, and they are initially growth delayed but eventually exceed the size of wild-type littermates. Mice at all ages show increased protein transport across the lung barrier; however, the phenotype is most discernible in 8-12-week-old male mice. When subjected to a clinically relevant LPS-induced lung injury model, 8-12-week-old young females and 30-36-week-old males seem to be the most significantly injured group. In contrast, 30-36-week-old males remain the most significantly injured group when mechanically ventilated at high tidal volumes, which is a clinically relevant model of mechanical stress lung injury. These data reveal that nmMLCK2 overexpression in the endothelium exacerbates lung injury in vivo in a sexually dimorphic and age-dependent manner.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.