ArticleNucleic acids research2008
Target accessibility and signal specificity in live-cell detection of BMP-4 mRNA using molecular beacons.
Article in Nucleic acids research, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
37 citing papers in PubMed.
- Molecular Dynamics Simulations of RNA Motifs to Guide the Architectural Parameters and Design Principles of RNA Nanostructures.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Live cell imaging of DNA and RNA with fluorescent signal amplification and background reduction techniques.Frontiers in cell and developmental biology · 2023Review
- RNA-Responsive gRNAs for Controlling CRISPR Activity: Current Advances, Future Directions, and Potential Applications.The CRISPR journal · 2022Review
- Recent Advances in the Molecular Beacon Technology for Live-Cell Single-Molecule Imaging.iScience · 2020Review
- Interrogation of highly structured RNA with multicomponent deoxyribozyme probes at ambient temperatures.RNA (New York, N.Y.) · 2020Article
- Nucleic-Acid Structures as Intracellular Probes for Live Cells.Advanced materials (Deerfield Beach, Fla.) · 2020Review
- Intracellular Delivery by Membrane Disruption: Mechanisms, Strategies, and Concepts.Chemical reviews · 2018Review
- Advancement of the Emerging Field of RNA Nanotechnology.ACS nano · 2017Review
- Efficient in situ detection of mRNAs using the Chlorella virus DNA ligase for padlock probe ligation.RNA (New York, N.Y.) · 2017Article
- Omics-based approaches to understand mechanosensitive endothelial biology and atherosclerosis.Wiley interdisciplinary reviews. Systems biology and medicine · 2016Review
- The role of endothelial mechanosensitive genes in atherosclerosis and omics approaches.Archives of biochemistry and biophysics · 2016Review
- Non-genetic Purification of Ventricular Cardiomyocytes from Differentiating Embryonic Stem Cells through Molecular Beacons Targeting IRX-4.Stem cell reports · 2015Article
- Use of siRNA molecular beacons to detect and attenuate mycobacterial infection in macrophages.World journal of experimental medicine · 2015Review
- Development of a universal RNA beacon for exogenous gene detection.Stem cells translational medicine · 2015Article
- Molecular beacon-enabled purification of living cells by targeting cell type-specific mRNAs.Nature protocols · 2014Article
- High expression of hTERT and stemness genes in BORIS/CTCFL positive cells isolated from embryonic cancer cells.PloS one · 2014Article
- Tracking neuronal marker expression inside living differentiating cells using molecular beacons.Frontiers in cellular neuroscience · 2013Article
- Purification of cardiomyocytes from differentiating pluripotent stem cells using molecular beacons that target cardiomyocyte-specific mRNA.Circulation · 2013Article
- Imaging mRNA expression levels in living cells with PNA·DNA binary FRET probes delivered by cationic shell-crosslinked nanoparticles.Organic & biomolecular chemistry · 2013Article
- SOX2 and OCT4 mRNA-expressing cells, detected by molecular beacons, localize to the center of neurospheres during differentiation.PloS one · 2013Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
The ability to visualize mRNA in single living cells and monitor in real-time the changes of mRNA level and localization can provide unprecedented opportunities for biological and disease studies. However, the mRNA detection specificity and sensitivity are critically dependent on the selection of target sequences and their accessibility. We carried out an extensive study of the target accessibility of BMP-4 mRNA using 10 different designs of molecular beacons (MBs), and identified the optimal beacon design. Specifically, for MB design 1 and 8 (MB1 and MB8), the fluorescent intensities from BMP-4 mRNA correlated well with the GFP signal after upregulating BMP-4 and co-expressing GFP using adenovirus, and the knockdown of BMP-4 mRNA using siRNA significantly reduced the beacon signals, demonstrating detection specificity. The beacon specificity was further confirmed using blocking RNA and in situ hybridization. We found that fluorescence signal from MBs depends critically on target sequences; the target sequences corresponding to siRNA sites may not be good sites for beacon-based mRNA detection, and vice versa. Possible beacon design rules are identified and approaches for enhancing target accessibility are discussed. This has significant implications to MB design for live cell mRNA detection.
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Registered trials
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