ArticleThe Journal of biological chemistry2008
Regulation of the Saccharomyces cerevisiae CKI1-encoded choline kinase by zinc depletion.
Article in The Journal of biological chemistry, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 26 citations in OpenAlex.
- The PAH1-encoded phosphatidate phosphatase of Yarrowia lipolytica differentially affects gene expression and lipid biosynthesis.Biochimica et biophysica acta. Molecular and cell biology of lipids · 2024Article
- The cellular economy of the Saccharomyces cerevisiae zinc proteome.Metallomics : integrated biometal science · 2018Article
- An Autophagy-Independent Role forGenetics · 2018Article
- The Zap1 transcriptional activator negatively regulates translation of the RTC4 mRNA through the use of alternative 5' transcript leaders.Molecular microbiology · 2017Article
- Transcription factor Reb1p regulates DGK1-encoded diacylglycerol kinase and lipid metabolism in Saccharomyces cerevisiae.The Journal of biological chemistry · 2013Article
- Phosphatidate phosphatase, a key regulator of lipid homeostasis.Biochimica et biophysica acta · 2013Review
- Review
- Phosphatidate phosphatase plays role in zinc-mediated regulation of phospholipid synthesis in yeast.The Journal of biological chemistry · 2012Article
- Regulation of phospholipid synthesis in the yeast Saccharomyces cerevisiae.Annual review of biochemistry · 2011Review
- Homeostatic and adaptive responses to zinc deficiency in Saccharomyces cerevisiae.The Journal of biological chemistry · 2009Review
- Regulation of phospholipid synthesis in yeast.Journal of lipid research · 2009Review
- The Kap60-Kap95 karyopherin complex directly regulates phosphatidylcholine synthesis.The Journal of biological chemistry · 2009Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
In the yeast Saccharomyces cerevisiae, the CKI1-encoded choline kinase catalyzes the committed step in the synthesis of phosphatidylcholine via the CDP-choline branch of the Kennedy pathway. Analysis of a P(CKI1)-lacZ reporter gene revealed that CKI1 expression was regulated by intracellular levels of the essential mineral zinc. Zinc depletion resulted in a concentration-dependent induction of CKI1 expression. This regulation was mediated by the zinc-sensing and zinc-inducible transcriptional activator Zap1p. A purified Zap1p probe interacted with two putative UAS(ZRE) sequences (ZRE1 and ZRE2) in the CKI1 promoter. Mutations of ZRE1 and ZRE2 to a nonconsensus UAS(ZRE) attenuated the induction of CKI1 expression in response to zinc depletion. A UAS(INO) element in the CKI1 promoter was responsible for stimulating CKI1 expression, but this element was not involved with the regulation by zinc depletion. The induction of CKI1 expression in zinc-depleted cells translated into increased choline kinase activity in vitro and in vivo, and an increase in phosphatidylcholine synthesis via the Kennedy pathway.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.