ArticleJournal of the American Society for Mass Spectrometry2008
Investigation of deprotonation reactions on globular and denatured proteins at atmospheric pressure by ESSI-MS.
Article in Journal of the American Society for Mass Spectrometry, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Predicting the Solubility of Amino Acids and Peptides with the SAFT-γ Mie Approach: Neutral and Charged Models.Industrial & engineering chemistry research · 2024Article
- Microdroplet Fusion Chemistry for Charge State Reduction of Synthetic Polymers via Bipolar Dual Spray with Anionic Reagents.Journal of the American Society for Mass Spectrometry · 2019Article
- Isolating Protein Charge State Reduction in Electrospray Droplets Using Femtosecond Laser Vaporization.Journal of the American Society for Mass Spectrometry · 2017Article
- Increasing protein charge state when using laser electrospray mass spectrometry.Journal of the American Society for Mass Spectrometry · 2015Article
- A computational model for protein ionization by electrospray based on gas-phase basicity.Journal of the American Society for Mass Spectrometry · 2012Article
- Investigating the role of adducts in protein supercharging with sulfolane.Journal of the American Society for Mass Spectrometry · 2012Article
- Vapor treatment of electrospray droplets: evidence for the folding of initially denatured proteins on the sub-millisecond time-scale.Journal of the American Society for Mass Spectrometry · 2012Article
- Negative electrospray droplet exposure to gaseous bases for the manipulation of protein charge state distributions.Analytical chemistry · 2011Article
- Electrospray droplet exposure to gaseous acids for the manipulation of protein charge state distributions.Analytical chemistry · 2010Article
- Effects of ion/ion proton transfer reactions on conformation of gas-phase cytochrome c ions.Journal of the American Society for Mass Spectrometry · 2010Article
- On the zwitterionic nature of gas-phase peptides and protein ions.PLoS computational biology · 2010Article
- Modifying the charge state distribution of proteins in electrospray ionization mass spectrometry by chemical derivatization.Journal of the American Society for Mass Spectrometry · 2009Article
- Increasing charge while preserving noncovalent protein complexes for ESI-MS.Journal of the American Society for Mass Spectrometry · 2009Article
Corrections and comments
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Authors and funding
3 authors.
Funding
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Abstract
Deprotonation reactions of multiply charged protein ions have been studied by introducing volatile reference bases at atmospheric pressure between an electrosonic spray ionization (ESSI) source and the inlet of a mass spectrometer. Apparent gas-phase basicities (GB(app)) of different charge states of protein ions were determined by a bracketing approach. The results obtained depend on the conformation of the protein ions in the gas phase, which is linked to the type of buffer used (denaturing or nondenaturing). In nondenaturing buffer, the GB(app) values are consistent with values predicted by the group of Kebarle using an electrostatic model (J. Mass Spectrom.2002, 38, 618) based on the crystal structures, but taking into account salt bridges between ionized basic and acidic sites on the protein surface. A new basicity order for the most basic sites was therefore obtained. An excellent agreement with the charge residue model (CRM) is obtained when comparing the observed and calculated maximum charge state. Decharging of the proteins in the electrosonic spray process could be also useful in the study on noncovalent complexes, by decreasing repulsive electrostatic interactions. A unified mechanism of the ESSI process is proposed.
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