ArticleThe Journal of clinical investigation2008
A contrast agent recognizing activated platelets reveals murine cerebral malaria pathology undetectable by conventional MRI.
Article in The Journal of clinical investigation, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
33 citing papers in PubMed, 92 citations in OpenAlex.
- Interventional real-time molecular MRI for targeting early myocardial injury in a pig model.Npj imaging · 2025Article
- Contribution of Magnetic Resonance Imaging Studies to the Understanding of Cerebral Malaria Pathogenesis.Pathogens (Basel, Switzerland) · 2024Review
- Diagnosis of cerebral malaria: Tools to reduceFrontiers in cellular and infection microbiology · 2023Review
- Tracking the immune response by MRI using biodegradable and ultrasensitive microprobes.Science advances · 2022Article
- Platelets as Mediators of Neuroinflammation and Thrombosis.Frontiers in immunology · 2020Review
- Extracellular vesicle integrins act as a nexus for platelet adhesion in cerebral microvessels.Scientific reports · 2019Article
- The Era of Thromboinflammation: Platelets Are Dynamic Sensors and Effector Cells During Infectious Diseases.Frontiers in immunology · 2019Review
- MRI demonstrates glutamine antagonist-mediated reversal of cerebral malaria pathology in mice.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Molecular Magnetic Resonance Imaging of Endothelial Activation in the Central Nervous System.Theranostics · 2018Review
- Covalent assembly of nanoparticles as a peptidase-degradable platform for molecular MRI.Nature communications · 2017Article
- A Unique Recombinant Fluoroprobe Targeting Activated Platelets AllowsTheranostics · 2017Article
- FeInternational journal of nanomedicine · 2017Article
- Molecular Imaging of Activated Platelets Allows the Detection of Pulmonary Embolism with Magnetic Resonance Imaging.Scientific reports · 2016Article
- Thrombus-Targeted Theranostic Microbubbles: A New Technology towards Concurrent Rapid Ultrasound Diagnosis and Bleeding-free Fibrinolytic Treatment of Thrombosis.Theranostics · 2016Article
- Particle generation, functionalization and sortase A-mediated modification with targeting of single-chain antibodies for diagnostic and therapeutic use.Nature protocols · 2015Article
- Molecular magnetic resonance imaging of angiogenesis in vivo using polyvalent cyclic RGD-iron oxide microparticle conjugates.Theranostics · 2015Article
- Real-time imaging reveals the dynamics of leukocyte behaviour during experimental cerebral malaria pathogenesis.PLoS pathogens · 2014Article
- In vivo imaging in NHP models of malaria: challenges, progress and outlooks.Parasitology international · 2014Review
- Imaging Plasmodium immunobiology in the liver, brain, and lung.Parasitology international · 2014Review
- Imaging Neuroinflammation - from Bench to Bedside.Journal of clinical & cellular immunology · 2014Article
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
Abstract
Human and murine cerebral malaria are associated with elevated levels of cytokines in the brain and adherence of platelets to the microvasculature. Here we demonstrated that the accumulation of platelets in the brain microvasculature can be detected with MRI, using what we believe to be a novel contrast agent, at a time when the pathology is undetectable by conventional MRI. Ligand-induced binding sites (LIBS) on activated platelet glycoprotein IIb/IIIa receptors were detected in the brains of malaria-infected mice 6 days after inoculation with Plasmodium berghei using microparticles of iron oxide (MPIOs) conjugated to a single-chain antibody specific for the LIBS (LIBS-MPIO). No binding of the LIBS-MPIO contrast agent was detected in uninfected animals. A combination of LIBS-MPIO MRI, confocal microscopy, and transmission electron microscopy revealed that the proinflammatory cytokine TNF-alpha, but not IL-1beta or lymphotoxin-alpha (LT-alpha), induced adherence of platelets to cerebrovascular endothelium. Peak platelet adhesion was found 12 h after TNF-alpha injection and was readily detected with LIBS-MPIO contrast-enhanced MRI. Temporal studies revealed that the level of MPIO-induced contrast was proportional to the number of platelets bound. Thus, the LIBS-MPIO contrast agent enabled noninvasive detection of otherwise undetectable cerebral pathology by in vivo MRI before the appearance of clinical disease, highlighting the potential of targeted contrast agents for diagnostic, mechanistic, and therapeutic studies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.