ArticlePlanta2008
Molecular cloning and functional characterization of alpha-humulene synthase, a possible key enzyme of zerumbone biosynthesis in shampoo ginger (Zingiber zerumbet Smith).
Article in Planta, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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25 citing papers in PubMed, 83 citations in OpenAlex.
- Pathway and efflux engineering to utilize endogenous high isoprenoid flux inApplied and environmental microbiology · 2026Article
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- Biologically Active Compounds of Malaysian Medicinal and Aromatic Plants.Progress in the chemistry of organic natural products · 2025Review
- Characterization of a sesquiterpene synthase and a short-chain dehydrogenase in zerumbone biosynthesis and the applications in engineeredFrontiers in plant science · 2025Article
- A Syringa pinnatifolia genome assembly reveals the zerumbone biosynthesis machinery with a cytochrome P450-catalysed epoxidation reaction in eudicots.Plant biotechnology journal · 2024Article
- Cloning, Expression and Functional Characterization of a Novel α-Humulene Synthase, Responsible for the Formation of Sesquiterpene in Agarwood Originating fromCurrent issues in molecular biology · 2023Article
- Molecular network of the oil palm root response to aluminum stress.BMC plant biology · 2023Article
- Deciphering chemical logic of fungal natural product biosynthesis through heterologous expression and genome mining.Natural product reports · 2023Review
- HarnessingJournal of natural products · 2022Review
- Chromosome-level genome assembly ofHorticulture research · 2022Article
- Understanding and Engineering the Stereoselectivity of Humulene Synthase.Angewandte Chemie (International ed. in English) · 2021Article
- A Hedychium coronarium short chain alcohol dehydrogenase is a player in allo-ocimene biosynthesis.Plant molecular biology · 2019Article
- Association Mapping Between Candidate Gene SNP and Production and Oil Quality Traits in Interspecific Oil Palm Hybrids.Plants (Basel, Switzerland) · 2019Article
- Identification of novel sesquiterpene synthase genes that mediate the biosynthesis of valerianol, which was an unknown ingredient of tea.Scientific reports · 2018Article
- Functional Characterisation of New Sesquiterpene Synthase from the Malaysian Herbal Plant,Molecules (Basel, Switzerland) · 2018Article
- Three previously unrecognised classes of biosynthetic enzymes revealed during the production of xenovulene A.Nature communications · 2018Article
- Bioproduction of α-humulene in metabolically engineeredEngineering in life sciences · 2017Article
- Overexpression ofPhysiology and molecular biology of plants : an international journal of functional plant biology · 2016Article
- Characterization of α-humulene synthases responsible for the production of sesquiterpenes induced by methyl jasmonate in Aquilaria cell culture.Journal of natural medicines · 2016Article
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Shampoo ginger (Zingiber zerumbet Smith) has a high content and large variety of terpenoids in the essential oil of its rhizome. Here, we report on the isolation of a cDNA clone (ZSS1) encoding alpha-humulene synthase, a possible key enzyme of zerumbone biosynthesis. This clone contains an open reading frame of 1,644 bp and is predicted to encode a protein of 548 amino acids with a calculated molecular mass of 64.5 kDa. The deduced amino acid sequence shows 34-63% identity with known sesquiterpene synthases of other angiosperm species. Based on exon-intron organization, ZSS1 is classified as the terpene synthase-III (TPS-III) subfamily. When expressed in Escherichia coli, the recombinant enzyme catalyzed the formation of a major product, alpha-humulene (95%) and a minor by-product, beta-caryophyllene (5%). Introduction of ZSS1 and the foreign mevalonate pathway involved in FPP synthesis into E. coli results in in vivo production of alpha-humulene. Transcript of ZSS1 was detected almost exclusively in rhizomes and was up-regulated in both leaves and rhizomes after treatment with methyl jasmonate (MeJA), suggesting its ecological function in shampoo ginger.
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