ArticleGene therapy2008
Increased virus replication in mammalian cells by blocking intracellular innate defense responses.
Article in Gene therapy, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed.
- Enhancing titers of therapeutic lentiviral vectors using PKC agonists.Molecular therapy. Methods & clinical development · 2025Article
- Impact of Influenza A Virus Infection on Growth and Metabolism of Suspension MDCK Cells Using a Dynamic Model.Metabolites · 2022Article
- Robust Enhancement of Lentivirus Production by Promoter Activation.Scientific reports · 2018Article
- Improving miRNA Delivery by Optimizing miRNA Expression Cassettes in Diverse Virus Vectors.Human gene therapy methods · 2017Review
- Tumor Restrictions to Oncolytic Virus.Biomedicines · 2014Review
- Aurintricarboxylic acid increases yield of HSV-1 vectors.Molecular therapy. Methods & clinical development · 2014Article
- Review
- Tumor-specific targeting with modified Sindbis viral vectors: evaluation with optical imaging and positron emission tomography in vivo.Molecular imaging and biology · 2013Article
- High yield production of influenza virus in Madin Darby canine kidney (MDCK) cells with stable knockdown of IRF7.PloS one · 2013Article
- The extent of sequence complementarity correlates with the potency of cellular miRNA-mediated restriction of HIV-1.Nucleic acids research · 2012Article
- Laem-Singh Virus: A Probable Etiological Agent Associated with Monodon Slow Growth Syndrome in Farmed Black Tiger Shrimp (Penaeus monodon).Indian journal of virology : an official organ of Indian Virological Society · 2012Article
- Article
- Viral RNAi suppressor reversibly binds siRNA to outcompete Dicer and RISC via multiple turnover.Journal of molecular biology · 2011Article
- Co-expression of miRNA targeting the expression of PERK, but not PKR, enhances cellular immunity from an HIV-1 Env DNA vaccine.PloS one · 2011Article
- Titers of lentiviral vectors encoding shRNAs and miRNAs are reduced by different mechanisms that require distinct repair strategies.RNA (New York, N.Y.) · 2010Article
- Metabolic effects of influenza virus infection in cultured animal cells: Intra- and extracellular metabolite profiling.BMC systems biology · 2010Article
- Foamy virus vectors for gene transfer.Expert opinion on biological therapy · 2009Review
- Human cellular restriction factors that target HIV-1 replication.BMC medicine · 2009Review
- The NS3 protein of rice hoja blanca virus complements the RNAi suppressor function of HIV-1 Tat.EMBO reports · 2009Article
- Human cellular microRNA hsa-miR-29a interferes with viral nef protein expression and HIV-1 replication.Retrovirology · 2008Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The mammalian innate immune system senses viral infection by recognizing viral signatures and activates potent antiviral responses. Besides the interferon (IFN) response, there is accumulating evidence that RNA silencing or RNA interference (RNAi) serves as an antiviral mechanism in mammalian cells. Mammalian viruses encode IFN antagonists to counteract the IFN response in infected cells. A number of IFN antagonists are also capable of blocking RNAi in infected cells and therefore serve as RNA-silencing suppressors. Virus replication in infected cells is restricted by these innate antiviral mechanisms, which may kick in earlier than the viral antagonistic or suppressor protein can accumulate. The yield of virus vaccines and viral gene delivery vectors produced in mammalian producer cells may therefore be suboptimal. To investigate whether blocking of the innate antiviral responses in mammalian cells leads to increased viral vector production, we expressed a number of immunity suppressors derived from plant and mammalian viruses in human cells. We measured that the yield of infectious human immunodeficiency virus-1 particles produced in these cells was increased 5- to 10-fold. In addition, the production of lentiviral and adenoviral vector particles was increased 5- to 10-fold, whereas Sindbis virus particle production was increased approximately 100-fold. These results can be employed for improving the production of viral gene transfer vectors and viral vaccine strains.
Indexed as
Identifiers
18273055What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.