ArticleAmerican journal of physiology. Renal physiology2008
Heterogeneity of muscarinic receptor-mediated Ca2+ responses in cultured urothelial cells from rat.
Article in American journal of physiology. Renal physiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed, 94 citations in OpenAlex.
- Review
- Aging-Associated Changes in Oxidative Stress Negatively Impacts the Urinary Bladder Urothelium.International neurourology journal · 2022Article
- Transient receptor potential vanilloid type 4 (TRPV4) in urinary bladder structure and function.Current topics in membranes · 2022Review
- The Urothelium: Life in a Liquid Environment.Physiological reviews · 2020Review
- Acute spinal cord injury is associated with mitochondrial dysfunction in mouse urothelium.Neurourology and urodynamics · 2019Article
- Stress-induced autonomic dysregulation of mitochondrial function in the rat urothelium.Neurourology and urodynamics · 2019Article
- Aging increases the expression of vasopressin receptors in both the kidney and urinary bladder.Neurourology and urodynamics · 2019Article
- Modulation of lower urinary tract smooth muscle contraction and relaxation by the urothelium.Naunyn-Schmiedeberg's archives of pharmacology · 2018Review
- The role of ATP signalling in response to mechanical stimulation studied in T24 cells using new microphysiological tools.Journal of cellular and molecular medicine · 2018Article
- Serotonergic paraneurones in the female mouse urethral epithelium and their potential role in peripheral sensory information processing.Acta physiologica (Oxford, England) · 2018Article
- Influence of urothelial or suburothelial cholinergic receptors on bladder reflexes in chronic spinal cord injured cats.Experimental neurology · 2016Article
- Effects of nicotinic receptor agonists on bladder afferent nerve activity in an in vitro bladder-pelvic nerve preparation.Brain research · 2016Article
- Three Gaseous Neurotransmitters, Nitric oxide, Carbon Monoxide, and Hydrogen Sulfide, Are Involved in the Neurogenic Relaxation Responses of the Porcine Internal Anal Sphincter.Journal of neurogastroenterology and motility · 2016Article
- Comparative functional selectivity of imidafenacin and propiverine, antimuscarinic agents, for the urinary bladder over colon in conscious rats.Naunyn-Schmiedeberg's archives of pharmacology · 2015Article
- Recovery of urothelial mediator release but prolonged elevations in interleukin-8 and nitric oxide secretion following mitomycin C treatment.Naunyn-Schmiedeberg's archives of pharmacology · 2015Article
- Expression of brain derived-neurotrophic factor and granulocyte-colony stimulating factor in the urothelium: relation with voiding function.BMC urology · 2015Article
- Neural control of the lower urinary tract.Comprehensive Physiology · 2015Review
- Combination of foot stimulation and tolterodine treatment eliminates bladder overactivity in cats.Neurourology and urodynamics · 2014Article
- Regulation of ACh release from guinea pig bladder urothelial cells: potential role in bladder filling sensations.British journal of pharmacology · 2014Article
- Human urothelial cell lines as potential models for studying cannabinoid and excitatory receptor interactions in the urinary bladder.Naunyn-Schmiedeberg's archives of pharmacology · 2014Article
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6 authors at 1 institution in 1 country.
Funding
Abstract
Muscarinic receptors (mAChRs) have been identified in the urothelium, a tissue that may be involved in bladder sensory mechanisms. This study investigates the expression and function of mAChRs using cultured urothelial cells from the rat. RT-PCR established the expression of all five mAChR subtypes. Muscarinic agonists acetylcholine (ACh; 10 microM), muscarine (Musc; 20 microM), and oxotremorine methiodide (OxoM; 0.001-20 microM) elicited transient repeatable increases in the intracellular calcium concentration ([Ca(2+)](i)) in approximately 50% of cells. These effects were blocked by the mAChR antagonist atropine methyl nitrate (10 microM). The sources of [Ca(2+)](i) changes included influx from external milieu in 63% of cells and influx from external milieu plus release from internal stores in 27% of cells. The use of specific agonists and antagonists (10 microM M(1) agonist McN-A-343; 10 microM M(2), M(3) antagonists AF-DX 116, 4-DAMP) revealed that M(1), M(2), M(3) subtypes were involved in [Ca(2+)](i) changes. The PLC inhibitor U-73122 (10 microM) abolished OxoM-elicited Ca(2+) responses in the presence of the M(2) antagonist AF-DX 116, suggesting that M(1), M(3), or M(5) mediates [Ca(2+)](i) increases via PLC pathway. ACh (0.1 microM), Musc (10 microM), oxotremorine sesquifumarate (20 microM), and McN-A-343 (1 muM) acting on M(1), M(2), and M(3) mAChR subtypes stimulated ATP release from cultured urothelial cells. In summary, cultured urothelial cells express functional M(1), M(2), and M(3) mAChR subtypes whose activation results in ATP release, possibly through mechanisms involving [Ca(2+)](i) changes.
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