ArticleThe Journal of biological chemistry2008
Stimulated efflux of amino acids and glutathione from cultured hippocampal slices by omission of extracellular calcium: likely involvement of connexin hemichannels.
Article in The Journal of biological chemistry, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 71 citations in OpenAlex.
- A metastasis-associated pannexin-1 mutant (Panx1The FEBS journal · 2025Article
- Arsenite-Mediated Transcriptional Regulation of Glutathione Synthesis in Mammalian Primary Cortical Astrocytes.International journal of molecular sciences · 2025Article
- Connexin43 Contributes to Alzheimer's Disease by Promoting the Mitochondria-Associated Membrane-Related Autophagy Inhibition.Molecular neurobiology · 2025Article
- Nrf2 Regulates Basal Glutathione Production in Astrocytes.International journal of molecular sciences · 2025Article
- Connexin hemichannels regulate redox potential via metabolite exchange and protect lens against cellular oxidative damage.Redox biology · 2021Article
- Connexin Gap Junctions and Hemichannels in Modulating Lens Redox Homeostasis and Oxidative Stress in Cataractogenesis.Antioxidants (Basel, Switzerland) · 2021Review
- Brain Disorders and Chemical Pollutants: A Gap Junction Link?Biomolecules · 2020Review
- Connexin Hemichannels Contribute to the Activation of cAMP Signaling Pathway and Renin Production.International journal of molecular sciences · 2020Article
- Contribution of Connexin Hemichannels to the Decreases in Cell Viability Induced by Linoleic Acid in the Human Lens Epithelial Cells (HLE-B3).Frontiers in physiology · 2019Article
- Interleukin-1β Protects Neurons against Oxidant-Induced Injury via the Promotion of Astrocyte Glutathione Production.Antioxidants (Basel, Switzerland) · 2018Article
- Connexin hemichannels mediate glutathione transport and protect lens fiber cells from oxidative stress.Journal of cell science · 2018Article
- Connexin Hemichannels in Astrocytes: An Assessment of Controversies Regarding Their Functional Characteristics.Neurochemical research · 2017Review
- Connexin43 Hemichannels in Satellite Glial Cells, Can They Influence Sensory Neuron Activity?Frontiers in molecular neuroscience · 2017Review
- New Implications for the Melanocortin System in Alcohol Drinking Behavior in Adolescents: The Glial Dysfunction Hypothesis.Frontiers in cellular neuroscience · 2017Article
- Connexin43 hemichannels contributes to the disassembly of cell junctions through modulation of intracellular oxidative status.Redox biology · 2016Article
- Astroglial connexin43 contributes to neuronal suffering in a mouse model of Alzheimer's disease.Cell death and differentiation · 2016Article
- Carbon Monoxide Modulates Connexin Function through a Lipid Peroxidation-Dependent Process: A Hypothesis.Frontiers in physiology · 2016Article
- Role of Astroglial Hemichannels and Pannexons in Memory and Neurodegenerative Diseases.Frontiers in integrative neuroscience · 2016Review
- Glutathione release through connexin hemichannels: Implications for chemical modification of pores permeable to large molecules.The Journal of general physiology · 2015Article
- Neuron-Glia Crosstalk in the Autonomic Nervous System and Its Possible Role in the Progression of Metabolic Syndrome: A New Hypothesis.Frontiers in physiology · 2015Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
Omission of extracellular Ca(2+) for 15 min from the incubation medium of cultured hippocampal slices stimulated the efflux of glutathione, phosphoethanolamine, hypotaurine, and taurine. The efflux was reduced by several blockers of gap junctions, i.e. carbenoxolone, flufenamic acid, and endothelin-1, and by the connexin43 hemichannel blocking peptide Gap26 but was unchanged by the P2X(7) receptor inhibitor oxidized ATP, a pannexin1 hemichannel blocking peptide and an inactive analogue of carbenoxolone. Pretreatment of the slices with the neurotoxin N-methyl-d -aspartate left the efflux by Ca(2+) omission unchanged, indicating that the stimulated efflux primarily originated from glia. Elevated glutamate efflux was detected when Ca(2+) omission was combined with the glutamate uptake blocker l-trans-pyrrolidine-2,4-dicarboxylate and when both Ca(2+) and Mg(2+) were omitted from the medium. Omission of Ca(2+) for 15 min alone did not induce delayed toxicity, but in combination with blocked glutamate uptake, significant cell death was observed 24 h later. Our results indicate that omission of extracellular Ca(2+) stimulates efflux of glutathione and specific amino acids including glutamate via opening of glial hemichannels. This type of efflux may have protective functions via glutathione efflux but can aggravate toxicity in situations when glutamate reuptake is impaired, such as following a stroke.
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Registered trials
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