ArticleSurgical endoscopy2008
CO(2) pneumoperitoneum increases systemic but not local tumor spread after intraperitoneal murine neuroblastoma spillage in mice.
Article in Surgical endoscopy, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 25 citations in OpenAlex.
- Beyond Sentinel Lymph Node: Outcomes of Indocyanine Green-Guided Pelvic Lymphadenectomy in Endometrial and Cervical Cancer.International journal of environmental research and public health · 2023Article
- Commentary on "Laparoscopic approach of pediatric adrenal tumors".Pediatric surgery international · 2023Article
- Effect of various hepatectomy procedures on circulating tumor cells in postoperative patients: a case-matched comparative study.Frontiers in medicine · 2023Article
- Minimally Invasive Surgery in Pediatric Surgical Oncology.Children (Basel, Switzerland) · 2018Review
- Maintaining oncologic integrity with minimally invasive resection of pediatric embryonal tumors.Surgery · 2018Article
- Contraindications and image-defined risk factors in laparoscopic resection of abdominal neuroblastoma.Pediatric surgery international · 2016Article
- The role of minimally invasive surgery in pediatric solid tumors.Pediatric surgery international · 2015Review
- Laparoscopic resection of adrenal neuroblastoma without image-defined risk factors: a prospective study on 21 consecutive pediatric patients.Pediatric surgery international · 2014Article
- Minimally invasive surgery for pediatric tumors - current state of the art.Frontiers in pediatrics · 2014Review
- In vitro CO2-induced ROS production impairs cell cycle in SH-SY5Y neuroblastoma cells.Pediatric surgery international · 2013Article
- Use of laparoscopic surgical resection for pediatric malignant solid tumors: a case series.Surgical endoscopy · 2011Article
- Carbon dioxide modifies the morphology and function of mesothelial cells and facilitates transepithelial neuroblastoma cell migration.Pediatric surgery international · 2010Article
- Carbon dioxide does not affect the methylation status of prognostic important oncogenes Rassf1A and DCR2 in neuroblastoma cells.Pediatric surgery international · 2008Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundMinimally invasive techniques are increasingly used for biopsy and resection of neuroblastoma, but the impact on the behavior of spilled tumor cells is unknown. We aimed to investigate whether CO(2) pneumoperitoneum can affect local or systemic tumor manifestation after spillage of neuroblastoma cells into the peritoneal cavity.
methodsMurine neuroblastoma cells (Neuro2a, 1x10(6)) were inoculated into the peritoneal cavity of 25 male A/J mice, which subsequently underwent CO(2) pneumoperitoneum (n = 12) or laparotomy (n = 13) for 1 h. At the 28th postoperative day, local (peritoneal and surface of the gut) and systemic (liver, lung, spine) tumor spread was graded in a blinded manner (1-4 point scale) and specimens were histologically examined for tumor manifestation (hematoxylin and eosin stain) and tumor cell proliferation rate (Ki-67-stain). In the case of no visible lesion, five random sections were histologically examined. Peritoneal carcinosis was graded macroscopically.
resultsTumor manifestations were detected in 10 out of 12 (83%) animals after CO(2) pneumoperitoneum, and in 9 out of 13 (69%) after laparotomy (n.s.). Incidence of liver metastasis was higher after CO(2) pneumoperitoneum versus laparotomy (83% versus 31%; p < 0.05). Incidence and grading of peritoneal carcinosis was not significantly different between the groups (n.s.). Intrapulmonary metastasis was found in one mouse of each group, but no metastasis of the spine. However, the grading of liver metastasis was higher after CO(2) pneumoperitoneum compared to laparotomy (p < 0.05). Tumor cell proliferation (Ki-67 stain) in the liver did not differ between both groups. Moreover, proliferation always exceeded 50% of tumor cells, irrespective local or systemic tumor manifestation.
conclusionsCO(2) pneumoperitoneum increased intrahepatic metastasis, but not local peritoneal carcinosis in a murine neuroblastoma model. This suggests that laparoscopy could promote systemic dissemination of intraperitoneally spilled tumor cells when no chemotherapy is applied. It remains to be determined whether this is due to local immune suppression or direct modulation of tumor cell behavior.
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