ArticleGraefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie2008
Expression profile of voltage-dependent Ca2+ channel subunits in the human retinal pigment epithelium.
Article in Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 24 citations in OpenAlex.
- Review of the clinical electrooculogram - Part 1: Mechanism of the Light-Rise.Documenta ophthalmologica. Advances in ophthalmology · 2026Review
- Exploring the potential for gene therapy in Cav1.4-related retinal channelopathies.Channels (Austin, Tex.) · 2025Review
- Ion channels research in hPSC-RPE cells: bridging benchwork to clinical applications.Journal of translational medicine · 2024Review
- Functional Voltage-Gated Calcium Channels Are Present in Human Embryonic Stem Cell-Derived Retinal Pigment Epithelium.Stem cells translational medicine · 2019Article
- Relevance of tissue specific subunit expression in channelopathies.Neuropharmacology · 2018Review
- Thrombin-Induced Calpain Activation Promotes Protease-Activated Receptor 1 Internalization.International journal of cell biology · 2017Article
- Ophthalmological assessment of cannabis-induced persisting perception disorder: is there a direct retinal effect?Documenta ophthalmologica. Advances in ophthalmology · 2015Article
- Expression of transient receptor potential vanilloid channels TRPV5 and TRPV6 in retinal pigment epithelium.Molecular vision · 2010Article
- The best disease-linked Cl- channel hBest1 regulates Ca V 1 (L-type) Ca2+ channels via src-homology-binding domains.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2008Article
- Voltage-dependent Ca2+ channels, not ryanodine receptors, activate Ca2+-dependent BK potassium channels in human retinal pigment epithelial cells.Molecular vision · 2008Article
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe secretion of a variety of factors by the retinal pigment epithelium (RPE) is essential for the structural integrity of the neuronal retina and choroid, but also plays a pivotal role in the etiology of diseases such as choroidal neovascularisation. A recent study showed that the secretory activity of the RPE is regulated by the activity of a certain type of voltage-dependent Ca(2+) channels, the L-type channel. In order to provide a better base for the understanding of the underlying Ca(2+) signalling in these cells, we investigated the expression profile of voltage-dependent Ca(2+) channel subunits in RPE cells.
methodsUsing RT-PCR techniques with cDNA isolated from RPE cells, we investigated the expression pattern of Ca(2+) channel subunits. Furthermore, we analysed Ba(2+) currents through voltage-dependent Ca(2+) channels in RPE cells by the patch-clamp technique.
resultsWe detected the expression of two L-type channel subtypes and the expression of two different T-type channel subtypes. As accessory subunits, they expressed beta2 and beta4 and all known alpha(2)delta subunits. In general, we were able to confirm these data with cDNA from the ARPE-19 cell line. They only showed some differences in their expression pattern of accessory subunits. Since the expression of T-type channels was so far unknown in RPE cells, we confirmed their expression in the RPE using cDNA isolated from freshly isolated human RPE cells. Furthermore, the patch-clamp analysis of Ba(2+) currents showed a heterogeneous pattern of voltage-dependent inward currents in RPE cells. In some cells, typical slowly inactivating L-type currents were detected, whereas in other cells fast inactivating T-type currents could be detected.
conclusionsThese data indicate the expression of a so far not detected subtype of voltage-dependent Ca(2+) channels, the T-type channels. Together with the expression of L-type channels, RPE cells show a comparable expression pattern to that of other secretory cells, such as beta-islets of the pancreas.
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