ArticleBMC biotechnology2008
High-throughput avian molecular sexing by SYBR green-based real-time PCR combined with melting curve analysis.
Article in BMC biotechnology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Molecular Tools for Non-invasive Sexing of Wildlife.Advances in experimental medicine and biology · 2026Review
- Cross-Species Validation of Pigeon-Specific CHD1 Primers for Molecular Sexing in Pet Birds.International journal of molecular sciences · 2025Article
- Application of Minimally Invasive Oral Swab Samples for qPCR-Based Sexing in Neognathae Birds.Veterinary sciences · 2025Article
- Comparison three primer pairs for molecular sex determination in Eurasian pygmy owls (Glaucidium passerinum).Scientific reports · 2024Article
- Fast and Simple Molecular Test for Sex Determination of the MonomorphicAnimals : an open access journal from MDPI · 2024Article
- Non-invasive sampling reveals low mitochondrial genetic diversity for an island endemic species: The critically endangered Grenada DoveEcology and evolution · 2023Article
- From maternal glucocorticoid and thyroid hormones to epigenetic regulation of offspring gene expression: An experimental study in a wild bird species.Evolutionary applications · 2023Article
- Prenatal thyroid hormones accelerate postnatal growth and telomere shortening in wild great tits.The Journal of experimental biology · 2023Article
- Effect of prenatal glucocorticoids and thyroid hormones on developmental plasticity of mitochondrial aerobic metabolism, growth and survival: an experimental test in wild great tits.The Journal of experimental biology · 2022Article
- Sexing chickens (Gallus gallus domesticus) with high-resolution melting analysis using feather crude DNA.Poultry science · 2021Article
- Testing for context-dependent effects of prenatal thyroid hormones on offspring survival and physiology: an experimental temperature manipulation.Scientific reports · 2020Article
- Simple, sensitive and robust chicken specific sexing assays, compliant with large scale analysis.PloS one · 2019Article
- Evaluation of the mRNA expression levels of integrins α3, α5, β1 and β6 as tumor biomarkers of oral squamous cell carcinoma.Oncology letters · 2018Article
- Condition-dependence of pheomelanin-based coloration in nuthatches Sitta europaea suggests a detoxifying function: implications for the evolution of juvenile plumage patterns.Scientific reports · 2017Article
- Barcoding melting curve analysis for rapid, sensitive, and discriminating authentication of saffron (Crocus sativus L.) from its adulterants.BioMed research international · 2014Article
- SNP 1772 C > T of HIF-1α gene associates with breast cancer risk in a Taiwanese population.Cancer cell international · 2014Article
- A quantitative real-time PCR method using an X-linked gene for sex typing in pigs.Molecular biotechnology · 2013Article
- Molecular sexing of threatened Gyps vultures: an important strategy for conservation breeding and ecological studies.SpringerPlus · 2012Article
- The mitogen-activated protein kinase phosphatase-1 (MKP-1) gene is a potential methylation biomarker for malignancy of breast cancer.Experimental & molecular medicine · 2012Article
- Real-time PCR machine system modeling and a systematic approach for the robust design of a real-time PCR-on-a-chip system.Sensors (Basel, Switzerland) · 2010Article
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11 authors.
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Abstract
backgroundCombination of CHD (chromo-helicase-DNA binding protein)-specific polymerase chain reaction (PCR) with electrophoresis (PCR/electrophoresis) is the most common avian molecular sexing technique but it is lab-intensive and gel-required. Gender determination often fails when the difference in length between the PCR products of CHD-Z and CHD-W genes is too short to be resolved.
resultsHere, we are the first to introduce a PCR-melting curve analysis (PCR/MCA) to identify the gender of birds by genomic DNA, which is gel-free, quick, and inexpensive. Spilornis cheela hoya (S. c. hoya) and Pycnonotus sinensis (P. sinensis) were used to illustrate this novel molecular sexing technique. The difference in the length of CHD genes in S. c. hoya and P. sinensis is 13-, and 52-bp, respectively. Using Griffiths' P2/P8 primers, molecular sexing failed both in PCR/electrophoresis of S. c. hoya and in PCR/MCA of S. c. hoya and P. sinensis. In contrast, we redesigned sex-specific primers to yield 185- and 112-bp PCR products for the CHD-Z and CHD-W genes of S. c. hoya, respectively, using PCR/MCA. Using this specific primer set, at least 13 samples of S. c. hoya were examined simultaneously and the Tm peaks of CHD-Z and CHD-W PCR products were distinguished.
conclusionIn this study, we introduced a high-throughput avian molecular sexing technique and successfully applied it to two species. This new method holds a great potential for use in high throughput sexing of other avian species, as well.
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