ArticleThe Journal of cell biology2008
Importin-beta and the small guanosine triphosphatase Ran mediate chromosome loading of the human chromokinesin Kid.
Article in The Journal of cell biology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 56 citations in OpenAlex.
- The chromosomal challenge of human embryos: Mechanisms and fundamentals.HGG advances · 2025Review
- Importin α/β promote Kif18B microtubule association and enhance microtubule destabilization activity.Molecular biology of the cell · 2023Article
- Pathogenic mutations in the chromokinesin KIF22 disrupt anaphase chromosome segregation.eLife · 2022Article
- Complementary functions for the Ran gradient during division.Small GTPases · 2021Review
- Aneuploidy in human eggs: contributions of the meiotic spindle.Biochemical Society transactions · 2021Review
- RanGTP induces an effector gradient of XCTK2 and importin α/β for spindle microtubule cross-linking.The Journal of cell biology · 2020Article
- High Expression of KIF22/Kinesin-Like DNA Binding Protein (Kid) as a Poor Prognostic Factor in Prostate Cancer Patients.Medical science monitor : international medical journal of experimental and clinical research · 2018Article
- NuSAP governs chromosome oscillation by facilitating the Kid-generated polar ejection force.Nature communications · 2016Article
- Review
- Regulatory mechanisms that control mitotic kinesins.Experimental cell research · 2015Review
- The Ran Pathway in Drosophila melanogaster Mitosis.Frontiers in cell and developmental biology · 2015Review
- The RanGTP Pathway: From Nucleo-Cytoplasmic Transport to Spindle Assembly and Beyond.Frontiers in cell and developmental biology · 2015Review
- Phosphorylation of nucleoporin Tpr governs its differential localization and is required for its mitotic function.Journal of cell science · 2014Article
- SENP1 and SENP2 affect spatial and temporal control of sumoylation in mitosis.Molecular biology of the cell · 2013Article
- Intrinsic Disorder in the Kinesin Superfamily.Biophysical reviews · 2013Article
- The molecular basis for kinesin functional specificity during mitosis.Cytoskeleton (Hoboken, N.J.) · 2013Review
- Identification of cargo proteins specific for the nucleocytoplasmic transport carrier transportin by combination of an in vitro transport system and stable isotope labeling by amino acids in cell culture (SILAC)-based quantitative proteomics.Molecular & cellular proteomics : MCP · 2013Article
- Kif18A and chromokinesins confine centromere movements via microtubule growth suppression and spatial control of kinetochore tension.Developmental cell · 2012Article
- Importin-β negatively regulates multiple aspects of mitosis including RANGAP1 recruitment to kinetochores.The Journal of cell biology · 2012Article
- Localization of Pom121 to the inner nuclear membrane is required for an early step of interphase nuclear pore complex assembly.Molecular biology of the cell · 2011Article
Corrections and comments
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Authors and funding
12 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nucleocytoplasmic transport factors mediate various cellular processes, including nuclear transport, spindle assembly, and nuclear envelope/pore formation. In this paper, we identify the chromokinesin human kinesin-like DNA binding protein (hKid) as an import cargo of the importin-alpha/beta transport pathway and determine its nuclear localization signals (NLSs). Upon the loss of its functional NLSs, hKid exhibited reduced interactions with the mitotic chromosomes of living cells. In digitonin-permeabilized mitotic cells, hKid was bound only to the spindle and not to the chromosomes themselves. Surprisingly, hKid bound to importin-alpha/beta was efficiently targeted to mitotic chromosomes. The addition of Ran-guanosine diphosphate and an energy source, which generates Ran-guanosine triphosphate (GTP) locally at mitotic chromosomes, enhanced the importin-beta-mediated chromosome loading of hKid. Our results indicate that the association of importin-beta and -alpha with hKid triggers the initial targeting of hKid to mitotic chromosomes and that local Ran-GTP-mediated cargo release promotes the accumulation of hKid on chromosomes. Thus, this study demonstrates a novel nucleocytoplasmic transport factor-mediated mechanism for targeting proteins to mitotic chromosomes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.