ArticleBMC molecular biology2008
Ligand-induced sequestering of branchpoint sequence allows conditional control of splicing.
Article in BMC molecular biology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 43 citations in OpenAlex.
- Enhancing small-molecule-mediated translational control through multivalent RNA aptamers.Nucleic acids research · 2026Article
- Riboswitches for Controlled Expression of Therapeutic Transgenes Delivered by Adeno-Associated Viral Vectors.Pharmaceuticals (Basel, Switzerland) · 2021Review
- Inducible nuclear import by TetR aptamer-controlled 3' splice site selection.RNA (New York, N.Y.) · 2021Article
- Robust gene expression control in human cells with a novel universal TetR aptamer splicing module.Nucleic acids research · 2019Article
- A small, portable RNA device for the control of exon skipping in mammalian cells.Nucleic acids research · 2018Article
- Mammalian synthetic biology for studying the cell.The Journal of cell biology · 2017Review
- Mammalian synthetic biology: emerging medical applications.Journal of the Royal Society, Interface · 2015Review
- Synthetic RNA switches as a tool for temporal and spatial control over gene expression.Current opinion in biotechnology · 2012Review
- Transgene regulation in plants by alternative splicing of a suicide exon.Nucleic acids research · 2012Article
- Engineering biological systems with synthetic RNA molecules.Molecular cell · 2011Review
- Evaluating target silencing by short hairpin RNA mediated by the group I intron in cultured mammalian cells.BMC biotechnology · 2011Article
- Aptamers for allosteric regulation.Nature chemical biology · 2011Review
- Engineering ligand-responsive RNA controllers in yeast through the assembly of RNase III tuning modules.Nucleic acids research · 2011Article
- Reprogramming cellular behavior with RNA controllers responsive to endogenous proteins.Science (New York, N.Y.) · 2010Article
- Frameworks for programming biological function through RNA parts and devices.Chemistry & biology · 2009Review
- Alternative splicing as a therapeutic target for human diseases.Methods in molecular biology (Clifton, N.J.) · 2009Article
- Altered splicing of CEACAM1 in breast cancer: identification of regulatory sequences that control splicing of CEACAM1 into long or short cytoplasmic domain isoforms.Molecular cancer · 2008Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundDespite tremendous progress in understanding the mechanisms of constitutive and alternative splicing, an important and widespread step along the gene expression pathway, our ability to deliberately regulate gene expression at this step remains rudimentary. The present study was performed to investigate whether a theophylline-dependent "splice switch" that sequesters the branchpoint sequence (BPS) within RNA-theophylline complex can regulate alternative splicing.
resultsWe constructed a series of pre-mRNAs in which the BPS was inserted within theophylline aptamer. We show that theophylline-induced sequestering of BPS inhibits pre-mRNA splicing both in vitro and in vivo in a dose-dependent manner. Several lines of evidence suggest that theophylline-dependent inhibition of splicing is highly specific, and thermodynamic stability of RNA-theophylline complex as well as the location of BPS within this complex affects the efficiency of splicing inhibition. Finally, we have constructed an alternative splicing model pre-mRNA substrate in which theophylline caused exon skipping both in vitro and in vivo, suggesting that a small molecule-RNA interaction can modulate alternative splicing.
conclusionThese findings provide the ability to control splicing pattern at will and should have important implications for basic, biotechnological, and biomedical research.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.