ArticleCurrent genetics2008
Gene deletion and allelic replacement in the filamentous fungus Podospora anserina.
Article in Current genetics, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed, 78 citations in OpenAlex.
- Fungal DNA damage repair: from model to driver of virulence and AMR.Bioscience reports · 2026Review
- NADPH Dehydrogenase Gene Regulates Energy Distribution and Fatty Acid Metabolism During Fruiting Body Formation in the Filamentous Fungus Podospora anserina.Microbial biotechnology · 2026Article
- Genetic differentiation in the MAT-proximal region is not sufficient for suppressing recombination in Podospora anserina.G3 (Bethesda, Md.) · 2025Article
- Organelle activity organized by the endoplasmic reticulum-mitochondria encounter structure -ERMES- is essential forMicrobial cell (Graz, Austria) · 2025Article
- het-B allorecognition in Podospora anserina is determined by pseudo-allelic interaction of genes encoding a HET and lectin fold domain protein and a PII-like protein.PLoS genetics · 2024Article
- Mutations inJournal of fungi (Basel, Switzerland) · 2024Article
- The peroxisome protein translocation machinery is developmentally regulated in the fungusMicrobiology spectrum · 2024Article
- Allorecognition genes drive reproductive isolation in Podospora anserina.Nature ecology & evolution · 2022Article
- Functional Characterization of the GATA-Type Transcription Factor PaNsdD in the Filamentous Fungus Podospora anserina and Its Interplay with the Sterigmatocystin Pathway.Applied and environmental microbiology · 2022Article
- Lifespan Increase ofCells · 2022Article
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- Important role of melanin for fertility in the fungus Podospora anserina.G3 (Bethesda, Md.) · 2021Article
- An Efficient Strategy for Obtaining Mutants by Targeted Gene Deletion inFrontiers in microbiology · 2021Article
- Involvement ofFrontiers in microbiology · 2020Article
- Distinct Contributions of the Peroxisome-Mitochondria Fission Machinery During Sexual Development of the FungusFrontiers in microbiology · 2020Article
- Article
- Combinations ofeLife · 2019Article
- NLR surveillance of essential SEC-9 SNARE proteins induces programmed cell death upon allorecognition in filamentous fungi.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Regulation of Aerobic Energy Metabolism in Podospora anserina by Two Paralogous Genes Encoding Structurally Different c-Subunits of ATP Synthase.PLoS genetics · 2016Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Gene replacement via homologous recombination is a fundamental tool for the analysis of gene function. However, this event is rare in organisms like the filamentous fungus Podospora anserina. We show here that deletion of the PaKu70 gene is an efficient strategy for improving gene manipulation in this organism. By using the DeltaPaKu70 strain, it is now possible (1) to produce deletion mutants with an efficiency of 100%, (2) to achieve allelic exchange by introducing a mutated allele associated with a selection cassette at the locus, (3) to introduce a mutation in a gene without co-insertion of a selectable marker and without any modification of the target locus.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.