ArticlePlant molecular biology2008
Agrobacterium-mediated transformation of maize (Zea mays) with Cre-lox site specific recombination cassettes in BIBAC vectors.
Article in Plant molecular biology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 54 citations in OpenAlex.
- Artificial chromosome technology and its potential application in plants.Frontiers in plant science · 2022Review
- Construction of Marker-Free Genetically Modified Maize Using a Heat-Inducible Auto-Excision Vector.Genes · 2019Article
- Generating Transgenic Plants with Single-copy Insertions Using BIBAC-GW Binary Vector.Journal of visualized experiments : JoVE · 2018Article
- Application of the Cre/loxP Site-Specific Recombination System for Gene Transformation in Aurantiochytrium limacinum.Molecules (Basel, Switzerland) · 2015Article
- Stable mitotic inheritance of rice minichromosomes in cell suspension cultures.Plant cell reports · 2015Article
- Transformation of rice with large maize genomic DNA fragments containing high content repetitive sequences.Plant cell reports · 2015Article
- Engineering of plant chromosomes.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2015Review
- Engineered minichromosomes in plants.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2015Review
- Advanced genetic tools for plant biotechnology.Nature reviews. Genetics · 2013Review
- Genomic resources for gene discovery, functional genome annotation, and evolutionary studies of maize and its close relatives.Genetics · 2013Article
- Telomere-mediated truncation of barley chromosomes.Chromosoma · 2012Article
- Towards the development of better crops by genetic transformation using engineered plant chromosomes.Plant cell reports · 2011Review
- A large insert Thellungiella halophila BIBAC library for genomics and identification of stress tolerance genes.Plant molecular biology · 2010Article
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Cre/loxP site-specific recombination system has been applied in various plant species including maize (Zea mays) for marker gene removal, gene targeting, and functional genomics. A BIBAC vector system was adapted for maize transformation with a large fragment of genetic material including a herbicide resistance marker gene, a 30 kb yeast genomic fragment as a marker for fluorescence in situ hybridization (FISH), and a 35S-lox-cre recombination cassette. Seventy-five transgenic lines were generated from Agrobacterium-mediated transformation of a maize Hi II line with multiple B chromosomes. Eighty-four inserts have been localized among all 10 A chromosome pairs by FISH using the yeast DNA probe together with a karyotyping cocktail. No inserts were found on the B chromosomes; thus a bias against the B chromosomes by the Agrobacterium-mediated transformation was revealed. The expression of a cre gene was confirmed in 68 of the 75 transgenic lines by a reporter construct for cre/lox mediated recombination. The placement of the cre/lox site-specific recombination system in many locations in the maize genome will be valuable materials for gene targeting and chromosome engineering.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.