ArticleThe Journal of membrane biology2008
Multiple KCNQ potassium channel subtypes mediate basal anion secretion from the human airway epithelial cell line Calu-3.
Article in The Journal of membrane biology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 41 citations in OpenAlex.
- Potentiation of BKCa channels by cystic fibrosis transmembrane conductance regulator correctors VX-445 and VX-121.The Journal of clinical investigation · 2024Article
- An electrochemical gram-scale protocol for pyridylation of inert N-heterocycles with cyanopyridines.STAR protocols · 2022Article
- Electrochemical ammonium-cation-assisted pyridylation of inert N-heterocycles via dual-proton-coupled electron transfer.iScience · 2022Article
- A Novel Role of Arrhythmia-Related GeneInternational journal of molecular sciences · 2022Article
- Dysregulated signalling pathways in innate immune cells with cystic fibrosis mutations.Cellular and molecular life sciences : CMLS · 2020Review
- Kv7 Channels in Lung Diseases.Frontiers in physiology · 2020Review
- KCNQ5 activation is a unifying molecular mechanism shared by genetically and culturally diverse botanical hypotensive folk medicines.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- The KCNE2 potassium channel β subunit is required for normal lung function and resilience to ischemia and reperfusion injury.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2019Article
- Investigating CFTR and KCa3.1 Protein/Protein Interactions.PloS one · 2016Article
- A spatial model of fluid recycling in the airways of the lung.Journal of theoretical biology · 2015Article
- KCNQ (Kv7) potassium channel activators as bronchodilators: combination with a β2-adrenergic agonist enhances relaxation of rat airways.American journal of physiology. Lung cellular and molecular physiology · 2014Article
- Article
- Pseudohalide anions reveal a novel extracellular site for potentiators to increase CFTR function.British journal of pharmacology · 2012Article
- A role for two-pore K⁺ channels in modulating Na⁺ absorption and Cl⁻ secretion in normal human bronchial epithelial cells.American journal of physiology. Lung cellular and molecular physiology · 2012Article
- KCNQ5/K(v)7.5 potassium channel expression and subcellular localization in primate retinal pigment epithelium and neural retina.American journal of physiology. Cell physiology · 2011Article
- Functional apical large conductance, Ca2+-activated, and voltage-dependent K+ channels are required for maintenance of airway surface liquid volume.The Journal of biological chemistry · 2011Article
- Ion transport by pulmonary epithelia.Journal of biomedicine & biotechnology · 2011Review
- Endolymphatic sodium homeostasis by extramacular epithelium of the saccule.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2009Article
- In situ measurement of airway surface liquid [K+] using a ratioable K+-sensitive fluorescent dye.The Journal of biological chemistry · 2009Article
- Regulation of ENaC-mediated sodium transport by glucocorticoids in Reissner's membrane epithelium.American journal of physiology. Cell physiology · 2009Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Potassium channels play an important role in providing a driving force for anion secretion from secretory epithelia. To investigate the role of KCNQ K+ channels in mediating rates of basal anion secretion across the human airway submucosal gland serous cell model, the Calu-3 cell, we examined the expression, localization and function of these channels. In addition to our previous knowledge that Calu-3 cells express KCNQ1, using reverse transcriptase polymerase chain reaction we determined expression of KCNQ3, KCNQ4 and KCNQ5 mRNA transcripts. Immunoblotting detected KCNQ1, KCNQ3 and KCNQ5 proteins, while KCNQ4 protein was not found. Immunolocalization using polarized Calu-3 cell monolayers revealed that KCNQ1 and KCNQ3 were located in or toward the apical membrane of the cells, while KCNQ5 was detected in the apical and lateral membranes. Transepithelial transport studies revealed a small chromanol 293B-sensitive current at the apical membrane, likely KCNQ1. Application of XE991, an inhibitor of all members of the KCNQ channel family, inhibited the basal short-circuit current when applied to both sides of the cells to a greater extent than 293B, with the largest inhibition seen upon apical application. This result was confirmed using linopiridine, a less potent analogue of XE991, and suggests that functional KCNQ3 and KCNQ5, in addition to KCNQ1, are present at the apical aspect of these cells. These results demonstrate the role of a number of KCNQ channel members in controlling basal anion secretion across Calu-3 cells, while also demonstrating the importance of apically located K+ channels in mediating anion secretion in the airway epithelium.
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