ArticlePlant physiology2008
Pollen tube growth oscillations and intracellular calcium levels are reversibly modulated by actin polymerization.
Article in Plant physiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 96 papers.
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Who cites it
96 citing papers in PubMed, 185 citations in OpenAlex.
- Plant cell surface receptors.The Plant journal : for cell and molecular biology · 2026Review
- Extracellular calcium modulates pollen tube growth and guidance inbioRxiv : the preprint server for biology · 2026Article
- Visualizing Pollen and Pollen Tube Development Using Fluorescence Microscopy.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Integrated RNA-seq and small RNA-seq profiling reveals tissue-associated transcriptional programs and candidate miRNA-mRNA relationships across tea floral tissues.Frontiers in plant science · 2026Article
- Inhibition of Sterol Biosynthesis Alters Tubulin Association with Detergent-Insoluble Membranes and Affects Microtubule Organization in Pollen Tubes ofPlants (Basel, Switzerland) · 2025Article
- Mechanosensitive ion channel MSL8 is required for oscillatory growth and cell wall dynamics in Arabidopsis pollen tubes.Plant reproduction · 2025Article
- A Plant-Derived Antifungal Agent, Poacic Acid, Inhibits Germination and Tube Growth of Lily Pollen.Plants (Basel, Switzerland) · 2025Article
- A land plant-specific VPS13 mediates polarized vesicle trafficking in germinating pollen.The New phytologist · 2025Article
- Multi-omics analysis of green lineage osmotic stress pathways unveils crucial roles of different cellular compartments.Nature communications · 2024Article
- Plant cell polarity: The many facets of sidedness.Plant physiology · 2023Article
- Movement of Lipid Droplets in the Arabidopsis Pollen Tube Is Dependent on the Actomyosin System.Plants (Basel, Switzerland) · 2023Article
- Sterols and Sphingolipids as New Players in Cell Wall Building and Apical Growth ofPlants (Basel, Switzerland) · 2022Article
- Let's shape again: the concerted molecular action that builds the pollen tube.Plant reproduction · 2022Review
- What are the key mechanisms that alter the morphology of stigmatic papillae inPlant signaling & behavior · 2021Review
- Apical pollen tube wall curvature correlates with growth and indicates localized changes in the yielding of the cell wall.Protoplasma · 2021Article
- Quantitative cell biology of tip growth in moss.Plant molecular biology · 2021Review
- An optimized genetically encoded dual reporter for simultaneous ratio imaging of CaThe New phytologist · 2021Article
- Protein Analysis of Pollen Tubes after the Treatments of Membrane Trafficking Inhibitors Gains Insights on Molecular Mechanism Underlying Pollen Tube Polar Growth.The protein journal · 2021Article
- Quantitative Structural Organization of Bulk Apical Membrane Traffic in Pollen Tubes.Plant physiology · 2020Article
- Exocytosis and endocytosis: coordinating and fine-tuning the polar tip growth domain in pollen tubes.Journal of experimental botany · 2020Review
36 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Prevention of actin polymerization with low concentrations of latrunculin B (Lat-B; 2 nm) exerts a profound inhibitory effect on pollen tube growth. Using flow-through chambers, we show that growth retardation starts after 10 min treatment with 2 nm Lat-B, and by 15 to 20 min reaches a basal rate of 0.1 to 0.2 microm/s, during which the pollen tube exhibits relatively few oscillations. If treated for 30 min, complete stoppage of growth can occur. Studies on the intracellular Ca(2+) concentration indicate that the tip-focused gradient declines in parallel with the inhibition of growth. Tubes exhibiting nonoscillating growth display a similarly reduced and nonoscillating Ca(2+) gradient. Studies on the pH gradient indicate that Lat-B eliminates the acidic domain at the extreme apex, and causes the alkaline band to move more closely to the tip. Removing Lat-B and returning the cells to control medium reverses these effects. Phalloidin staining of F-actin reveals that 2 nm Lat-B degrades the cortical fringe; it also disorganizes the microfilaments in the shank causing the longitudinally oriented elements to be disposed in swirls. Cytoplasmic streaming continues under these conditions, however the clear zone is obliterated with all organelles moving into and through the extreme apex of the tube. We suggest that actin polymerization promotes pollen tube growth through extension of the cortical actin fringe, which serves as a track to target cell wall vesicles to preferred exocytotic sites on the plasma membrane.
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