ArticleApplied and environmental microbiology2008
Cultivation-independent characterization of methylobacterium populations in the plant phyllosphere by automated ribosomal intergenic spacer analysis.
Article in Applied and environmental microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
- Lanthanide-dependent isolation of phyllosphere methylotrophs selects for a phylogenetically conserved but metabolically diverse community.bioRxiv : the preprint server for biology · 2025Article
- Glycine betaine metabolism is enabled inApplied and environmental microbiology · 2024Article
- Biosynthesis of polyhydroxybutyrate byFrontiers in plant science · 2024Article
- Diversity of Methylobacterium species associated with New Zealand native plants.FEMS microbiology letters · 2023Article
- Capacity of soil bacteria to reach the phyllosphere and convergence of floral communities despite soil microbiota variation.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Comprehensive Comparative Genomics and Phenotyping ofFrontiers in microbiology · 2021Article
- Article
- Mitigation of Salinity Stress in Wheat Seedlings Due to the Application of Phytohormone-Rich Culture Filtrate Extract of Methylotrophic ActinobacteriumFrontiers in microbiology · 2020Article
- Seed Endophyte Microbiome of Crotalaria pumila Unpeeled: Identification of Plant-Beneficial Methylobacteria.International journal of molecular sciences · 2018Article
- Correlated production and consumption of chloromethane in the Arabidopsis thaliana phyllosphere.Scientific reports · 2017Article
- Bacterial and diazotrophic diversities of endophytes in Dendrobium catenatum determined through barcoded pyrosequencing.PloS one · 2017Article
- Leaf-FISH: Microscale Imaging of Bacterial Taxa on Phyllosphere.Frontiers in microbiology · 2017Article
- Systems-level Proteomics of Two Ubiquitous Leaf Commensals Reveals Complementary Adaptive Traits for Phyllosphere Colonization.Molecular & cellular proteomics : MCP · 2016Article
- Effects of Elevated Tropospheric Ozone Concentration on the Bacterial Community in the Phyllosphere and Rhizoplane of Rice.PloS one · 2016Article
- Article
- Prerequisites for amplicon pyrosequencing of microbial methanol utilizers in the environment.Frontiers in microbiology · 2013Review
- Eukaryotic diversity in premise drinking water using 18S rDNA sequencing: implications for health risks.Environmental science and pollution research international · 2013Article
- Isolation of optically targeted single bacteria by application of fluidic force microscopy to aerobic anoxygenic phototrophs from the phyllosphere.Applied and environmental microbiology · 2013Article
- High-throughput identification and screening of novel Methylobacterium species using whole-cell MALDI-TOF/MS analysis.PloS one · 2012Article
- A catalytic role of XoxF1 as La3+-dependent methanol dehydrogenase in Methylobacterium extorquens strain AM1.PloS one · 2012Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bacteria of the genus Methylobacterium are widespread in the environment, but their ecological role in ecosystems, such as the plant phyllosphere, is not very well understood. To gain better insight into the distribution of different Methylobacterium species in diverse ecosystems, a rapid and specific cultivation-independent method for detection of these organisms and analysis of their community structure is needed. Therefore, 16S rRNA gene-targeted primers specific for this genus were designed and evaluated. These primers were used in PCR in combination with a reverse primer that binds to the tRNA(Ala) gene, which is located upstream of the 23S rRNA gene in the 16S-23S intergenic spacer (IGS). PCR products that were of different lengths were obtained due to the length heterogeneity of the IGS of different Methylobacterium species. This length variation allowed generation of fingerprints of Methylobacterium communities in environmental samples by automated ribosomal intergenic spacer analysis. The Methylobacterium communities on leaves of different plant species in a natural field were compared using this method. The new method allows rapid comparisons of Methylobacterium communities and is thus a useful tool to study Methylobacterium communities in different ecosystems.
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