ArticleApplied and environmental microbiology2008
Markerless multiple-gene-deletion system for Streptococcus mutans.
Article in Applied and environmental microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 48 citations in OpenAlex.
- Multiple genes deletion based on Cre-loxP marker-less gene deletion system for the strains from the genus of Pectobacterium.Biotechnology letters · 2024Article
- Illuminating the oral microbiome and its host interactions: tools and approaches for molecular microbiology studies.FEMS microbiology reviews · 2023Review
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- Synthesis of a Novel Lantibiotic Using Mutacin II Biosynthesis Apparatus.Microbiology spectrum · 2023Article
- ClpX/P-Dependent Degradation of Novel Substrates in Streptococcus mutans.Journal of bacteriology · 2022Article
- The W-Acidic Motif of Histidine Kinase WalK Is Required for Signaling and Transcriptional Regulation inFrontiers in microbiology · 2022Article
- Induction ofMicrobiology (Reading, England) · 2020Article
- Role of CovR phosphorylation in gene transcription in Streptococcus mutans.Microbiology (Reading, England) · 2018Article
- Pleiotropic Regulation of Virulence Genes in Streptococcus mutans by the Conserved Small Protein SprV.Journal of bacteriology · 2017Article
- SepM, a Streptococcal Protease Involved in Quorum Sensing, Displays Strict Substrate Specificity.Journal of bacteriology · 2016Article
- Degradation of SsrA-tagged proteins in streptococci.Microbiology (Reading, England) · 2015Article
- Article
- Cloning-independent plasmid construction for genetic studies in streptococci.Journal of microbiological methods · 2013Article
- Streptococcus mutans: a new Gram-positive paradigm?Microbiology (Reading, England) · 2013Review
- An extracelluar protease, SepM, generates functional competence-stimulating peptide in Streptococcus mutans UA159.Journal of bacteriology · 2012Article
- SMU.152 acts as an immunity protein for mutacin IV.Journal of bacteriology · 2012Article
- CtsR regulation in mcsAB-deficient Gram-positive bacteria.Journal of bacteriology · 2012Article
- Regulation of transcription by SMU.1349, a TetR family regulator, in Streptococcus mutans.Journal of bacteriology · 2011Article
- Generalized schemes for high-throughput manipulation of the Desulfovibrio vulgaris genome.Applied and environmental microbiology · 2011Article
- Cloning-independent and counterselectable markerless mutagenesis system in Streptococcus mutans.Applied and environmental microbiology · 2011Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Inactivation or selective modification is essential to elucidate the putative function of a gene. The present study describes an improved Cre-loxP-based method for markerless multiple gene deletion in Streptococcus mutans, the principal etiological agent of dental caries. This modified method uses two mutant loxP sites, which after recombination creates a double-mutant loxP site that is poorly recognized by Cre recombinase, facilitating multiple gene deletions in a single genetic background. The effectiveness of this modified strategy was demonstrated by the construction of both single and double gene deletions at the htrA and clpP loci on the chromosome of Streptococcus mutans. HtrA and ClpP play key roles in the processing and maturation of several important proteins, including many virulence factors. Deletion of these genes resulted in reducing the organism's ability to withstand exposure to low pH and oxidative agents. The method described here is simple and efficient and can be easily implemented for multiple gene deletions with S. mutans and other streptococci.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.