ArticleProceedings of the National Academy of Sciences of the United States of America2008
Rapid selection of accessible and cleavable sites in RNA by Escherichia coli RNase P and random external guide sequences.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Ribozyme-Mediated Downregulation Uncovers DNA Integrity Scanning Protein A (DisA) as a Solventogenesis Determinant inFrontiers in bioengineering and biotechnology · 2021Article
- Targeting protein translation, RNA splicing, and degradation by morpholino-based conjugates in Plasmodium falciparum.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- External guide sequence technology: a path to development of novel antimicrobial therapeutics.Annals of the New York Academy of Sciences · 2015Review
- (Non-)translational medicine: targeting bacterial RNA.Frontiers in genetics · 2013Article
- Basic peptide-morpholino oligomer conjugate that is very effective in killing bacteria by gene-specific and nonspecific modes.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Inactivation of expression of two genes in Saccharomyces cerevisiae with the external guide sequence methodology.RNA (New York, N.Y.) · 2011Article
- Small molecule inhibitors of Staphylococcus aureus RnpA alter cellular mRNA turnover, exhibit antimicrobial activity, and attenuate pathogenesis.PLoS pathogens · 2011Article
- Synthetic circuits, devices and modules.Protein & cell · 2010Review
- Morpholino oligonucleotides do not participate perfectly in standard Watson-Crick complexes with RNA.RNA (New York, N.Y.) · 2010Article
- Of proteins and RNA: the RNase P/MRP family.RNA (New York, N.Y.) · 2010Review
- Inactivation of expression of several genes in a variety of bacterial species by EGS technology.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Inhibition of expression of virulence genes of Yersinia pestis in Escherichia coli by external guide sequences and RNase P.RNA (New York, N.Y.) · 2008Article
- Article
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A method of inhibiting the expression of particular genes by using external guide sequences (EGSs) has been improved in its rapidity and specificity. Random EGSs that have 14-nt random sequences are used in the selection procedure for an EGS that attacks the mRNA for a gene in a particular location. A mixture of the random EGSs, the particular target RNA, and RNase P is used in the diagnostic procedure, which, after completion, is analyzed in a gel with suitable control lanes. Within a few hours, the procedure is complete. The action of EGSs designed by an older method is compared with EGSs designed by the random EGS method on mRNAs from two bacterial pathogens.
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Registered trials
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