ArticleBiophysical journal2008
Determination of zeta-potential in rat organotypic hippocampal cultures.
Article in Biophysical journal, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 28 citations in OpenAlex.
- Numerical simulation study of nanoparticle diffusion in gray matter.Computational and structural biotechnology journal · 2024Article
- Electrokinetic convection-enhanced delivery for infusion into the brain from a hydrogel reservoir.Communications biology · 2024Article
- Electroosmotic Perfusion, External Microdialysis: Simulation and Experiment.ACS chemical neuroscience · 2023Article
- Effect of Particle Size and Surface Charge on Nanoparticles Diffusion in the Brain White Matter.Pharmaceutical research · 2022Article
- Designing electrode configuration of electroosmosis based edema treatment as a complement to hyperosmotic therapy.Acta neurochirurgica · 2021Article
- Influence of Anisotropic White Matter on Electroosmotic Flow Induced by Direct Current.Frontiers in bioengineering and biotechnology · 2021Article
- Electroosmotic Perfusion-Microdialysis Probe Created by Direct Laser Writing for Quantitative Assessment of Leucine Enkephalin Hydrolysis by Insulin-Regulated Aminopeptidase in Vivo.Analytical chemistry · 2020Article
- Electrokinetic Convection-Enhanced Delivery of Solutes to the Brain.ACS chemical neuroscience · 2020Article
- Electrokinetic infusions into hydrogels and brain tissue: Control of direction and magnitude of solute delivery.Journal of neuroscience methods · 2019Article
- Direct current stimulation of endothelial monolayers induces a transient and reversible increase in transport due to the electroosmotic effect.Scientific reports · 2018Article
- Numerical Modeling of Electroosmotic Push-Pull Perfusion and Assessment of Its Application to Quantitative Determination of Enzymatic Activity in the Extracellular Space of Mammalian Tissue.Analytical chemistry · 2017Article
- Electroosmotic perfusion of tissue: sampling the extracellular space and quantitative assessment of membrane-bound enzyme activity in organotypic hippocampal slice cultures.Analytical and bioanalytical chemistry · 2014Review
- Assessment of tissue viability following electroosmotic push-pull perfusion from organotypic hippocampal slice cultures.ACS chemical neuroscience · 2013Article
- Electroosmotic push-pull perfusion: description and application to qualitative analysis of the hydrolysis of exogenous galanin in organotypic hippocampal slice cultures.ACS chemical neuroscience · 2013Article
- Iontophoresis from a micropipet into a porous medium depends on the ζ-potential of the medium.Analytical chemistry · 2012Article
- Synthesis and characterization of a hydrogel with controllable electroosmosis: a potential brain tissue surrogate for electrokinetic transport.Langmuir : the ACS journal of surfaces and colloids · 2011Article
- A simple method for measuring organotypic tissue slice culture thickness.Journal of neuroscience methods · 2011Article
- Multi-scale study of nanoparticle transport and deposition in tissues during an injection process.Medical & biological engineering & computing · 2010Article
- Minimizing tissue damage in electroosmotic sampling.Analytical chemistry · 2010Article
- Electroosmotic sampling. Application to determination of ectopeptidase activity in organotypic hippocampal slice cultures.Analytical chemistry · 2010Article
Corrections and comments
- Erratum issued
Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
Abstract
zeta-potentials of entities such as cells and synaptosomes have been determined, but zeta of brain tissue has never been measured. Electroosmotic flow, and the resulting transport of neuroactive substances, would result from naturally occurring and experimentally or clinically induced electric fields if zeta is significant. We have developed a simple method for determining zeta in tissue. An electric field applied across a rat organotypic hippocampal slice culture (OHSC) drives fluorescent molecules through the tissue by both electroosmotic flow and electrophoresis. Fluorescence microscopy is used to determine each molecule's velocity. Independently, capillary electrophoresis is used to measure the molecules' electrophoretic mobilities. The experiment yields zeta-potential and average tissue tortuosity. The zeta-potential of OHSCs is -22 +/- 2 mV, and the average tortuosity is 1.83 +/- 0.06. In a refined experiment, zeta-potential is measured in various subregions. The zeta-potentials of the CA1 stratum pyramidale, CA3 stratum pyramidal, and dentate gyrus are -25.1 +/- 1.6 mV, -20.3 +/- 1.7 mV, and -25.4 +/- 1.0 mV, respectively. Simple dimensional arguments show that electroosmotic flow is potentially as important as diffusion in molecular transport.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.