ArticleNucleic acids research2008
A new class of cleavable fluorescent nucleotides: synthesis and optimization as reversible terminators for DNA sequencing by synthesis.
Article in Nucleic acids research, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
44 citing papers in PubMed.
- The Evolution of Next-Generation Sequencing Technologies.Methods in molecular biology (Clifton, N.J.) · 2025Review
- Applying nanopore sequencing in the etiological diagnosis of bloodstream infection.Frontiers in microbiology · 2025Review
- Controlled enzymatic synthesis of oligonucleotides.Communications chemistry · 2024Review
- Advances of research in diabetic cardiomyopathy: diagnosis and the emerging application of sequencing.Frontiers in cardiovascular medicine · 2024Review
- The chemistry of next-generation sequencing.Nature biotechnology · 2023Review
- Rapid Detection of SARS-CoV-2 Variants of Concern by Genomic Surveillance Techniques.Advances in experimental medicine and biology · 2023Article
- Sequence-dependent quenching of fluorescein fluorescence on single-stranded and double-stranded DNA.RSC advances · 2022Article
- Genomic Surveillance for Monitoring Variants of Concern: SARS-CoV-2 Delta, Omicron, and Beyond.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Denitrifying bacterial communities in surface-flow constructed wetlands during different seasons: characteristics and relationships with environment factors.Scientific reports · 2021Article
- The New Omics Era into Systems Approaches: What Is the Importance of Separation Techniques?Advances in experimental medicine and biology · 2021Article
- Application of Genomic Big Data in Plant Breeding:Past, Present, and Future.Plants (Basel, Switzerland) · 2020Review
- Impact of Nucleic Acid Sequencing on Viroid Biology.International journal of molecular sciences · 2020Review
- Bioinformatics Strategies, Challenges, and Opportunities for Next Generation Sequencing-Based HLA Genotyping.Transfusion medicine and hemotherapy : offizielles Organ der Deutschen Gesellschaft fur Transfusionsmedizin und Immunhamatologie · 2019Review
- Article
- Molecular techniques for the personalised management of patients with chronic myeloid leukaemia.Biomolecular detection and quantification · 2017Review
- A Review on the Applications of Next Generation Sequencing Technologies as Applied to Food-Related Microbiome Studies.Frontiers in microbiology · 2017Review
- Next generation sequencing technology and genomewide data analysis: Perspectives for retinal research.Progress in retinal and eye research · 2016Review
- Second-generation non-invasive high-throughput DNA sequencing technology in the screening of Down's syndrome in advanced maternal age women.Biomedical reports · 2016Article
- The sequence of sequencers: The history of sequencing DNA.Genomics · 2016Review
- Artificially designed pathogens - a diagnostic option for future military deployments.Military Medical Research · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fluorescent 2'-deoxynucleotides containing a protecting group at the 3'-O-position are reversible terminators enabling array-based DNA sequencing by synthesis (SBS) approaches. Herein, we describe the synthesis of a new family of 3'-OH unprotected cleavable fluorescent 2'-deoxynucleotides and their evaluation as reversible terminators for high-throughput DNA SBS strategies. In this first version, all four modified nucleotides bearing a cleavable disulfide Alexa Fluor(R) 594 dye were assayed for their ability to act as a reversible stop for the incorporation of the next labeled base. Their use in SBS leaded to a signal-no signal output after successive addition of each labeled nucleotide during the sequencing process (binary read-out). Solid-phase immobilized synthetic DNA target sequences were used to optimize the method that has been applied to DNA polymerized colonies or clusters obtained by in situ solid-phase amplification of fragments of genomic DNA templates.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.