ArticleThe Journal of biological chemistry2008
Single-stranded DNA-binding protein in vitro eliminates the orientation-dependent impediment to polymerase passage on CAG/CTG repeats.
Article in The Journal of biological chemistry, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed, 24 citations in OpenAlex.
- Slippage reconfiguration of trinucleotide repeat hairpins impedes resolution by human replication protein A.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- The mechanism of replication stalling and recovery within repetitive DNA.Nature communications · 2022Article
- Long-read sequencing technology indicates genome-wide effects of non-B DNA on polymerization speed and error rate.Genome research · 2018Article
- Sequence motifs capable of forming DNA stem-loop structures act as a replication diode.FEBS open bio · 2017Article
- The DNA replication program is altered at the FMR1 locus in fragile X embryonic stem cells.Molecular cell · 2014Article
- Exome-wide somatic microsatellite variation is altered in cells with DNA repair deficiencies.PloS one · 2014Article
- Inhibition of DNA synthesis facilitates expansion of low-complexity repeats: is strand slippage stimulated by transient local depletion of specific dNTPs?BioEssays : news and reviews in molecular, cellular and developmental biology · 2013Article
- Flap endonuclease 1.Annual review of biochemistry · 2013Review
- Altered replication in human cells promotes DMPK (CTG)(n) · (CAG)(n) repeat instability.Molecular and cellular biology · 2012Article
- Instability of (CTG)n•(CAG)n trinucleotide repeats and DNA synthesis.Cell & bioscience · 2012Article
- 5'CAG and 5'CTG Repeats Create Differential Impediment to the Progression of a Minimal Reconstituted T4 Replisome Depending on the Concentration of dNTPs.Molecular biology international · 2011Article
- Mechanisms of trinucleotide repeat instability during human development.Nature reviews. Genetics · 2010Review
- Replication-dependent instability at (CTG) x (CAG) repeat hairpins in human cells.Nature chemical biology · 2010Article
- Mechanistic studies of hairpin to duplex conversion for trinucleotide repeat sequences.The Journal of biological chemistry · 2010Article
- Coordination between polymerase beta and FEN1 can modulate CAG repeat expansion.The Journal of biological chemistry · 2009Article
- Whole-genome analyses reveal genetic instability of Acetobacter pasteurianus.Nucleic acids research · 2009Article
- Hijacking of the mismatch repair system to cause CAG expansion and cell death in neurodegenerative disease.DNA repair · 2008Review
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
Abstract
Small insertions and deletions of trinucleotide repeats (TNRs) can occur by polymerase slippage and hairpin formation on either template or newly synthesized strands during replication. Although not predicted by a slippage model, deletions occur preferentially when 5'-CTG is in the lagging strand template and are highly favored over insertion events in rapidly replicating cells. The mechanism for the deletion bias and the orientation dependence of TNR instability is poorly understood. We report here that there is an orientation-dependent impediment to polymerase progression on 5'-CAG and 5'-CTG repeats that can be relieved by the binding of single-stranded DNA-binding protein. The block depends on the primary sequence of the TNR but does not correlate with the thermodynamic stability of hairpins. The orientation-dependent block of polymerase passage is the strongest when 5'-CAG is the template. We propose a "template-push" model in which the slow speed of DNA polymerase across the 5'-CAG leading strand template creates a threat to helicase-polymerase coupling. To prevent uncoupling, the TNR template is pushed out and by-passed. Hairpins do not cause the block, but appear to occur as a consequence of polymerase pass-over.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.