ArticleBiochemistry2008
Characterization of heparin oligosaccharides binding specifically to antithrombin III using mass spectrometry.
Article in Biochemistry, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
24 citing papers in PubMed, 55 citations in OpenAlex.
- Ultra-high-performance liquid chromatography charge transfer dissociation mass spectrometry (UHPLC-CTD-MS) as a tool for analyzing the structural heterogeneity in carrageenan oligosaccharides.Analytical and bioanalytical chemistry · 2022Article
- Salt-free fractionation of complex isomeric mixtures of glycosaminoglycan oligosaccharides compatible with ESI-MS and microarray analysis.Scientific reports · 2019Article
- Integrated Approach to Identify Heparan Sulfate Ligand Requirements of Robo1.Journal of the American Chemical Society · 2016Article
- Article
- Control of organization and function of muscle and tendon by thrombospondin-4.Matrix biology : journal of the International Society for Matrix Biology · 2014Article
- Analysis of glycosaminoglycan-derived, precolumn, 2-aminoacridone-labeled disaccharides with LC-fluorescence and LC-MS detection.Nature protocols · 2014Article
- An approach for separation and complete structural sequencing of heparin/heparan sulfate-like oligosaccharides.Analytical chemistry · 2013Article
- Glycosaminoglycan glycomics using mass spectrometry.Molecular & cellular proteomics : MCP · 2013Review
- Top-down approach for the direct characterization of low molecular weight heparins using LC-FT-MS.Analytical chemistry · 2012Article
- Article
- LC-MS(n) analysis of isomeric chondroitin sulfate oligosaccharides using a chemical derivatization strategy.Journal of the American Society for Mass Spectrometry · 2011Article
- Highly sulfated nonreducing end-derived heparan sulfate domains bind fibroblast growth factor-2 with high affinity and are enriched in biologically active fractions.The Journal of biological chemistry · 2011Article
- Glycomics analysis of mammalian heparan sulfates modified by the human extracellular sulfatase HSulf2.PloS one · 2011Article
- Targeted analysis of glycomics liquid chromatography/mass spectrometry data.Analytical and bioanalytical chemistry · 2011Article
- Hyphenated techniques for the analysis of heparin and heparan sulfate.Analytical and bioanalytical chemistry · 2011Review
- Mass spectrometry and glycomics.Omics : a journal of integrative biology · 2010Review
- Screening for anticoagulant heparan sulfate octasaccharides and fine structure characterization using tandem mass spectrometry.Biochemistry · 2010Article
- Improved hydrophilic interaction chromatography LC/MS of heparinoids using a chip with postcolumn makeup flow.Analytical chemistry · 2010Article
- UV-MALDI-TOF mass spectrometry analysis of heparin oligosaccharides obtained by nitrous acid controlled degradation and high performance anion exchange chromatography.Journal of the American Society for Mass Spectrometry · 2010Article
- Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Heparan sulfate (HS) is a sulfated glycosaminoglycan attached to a core protein on the cell surface. Protein binding to cell surface HS is a key regulatory event for many cellular processes such as blood coagulation, cell proliferation, and migration. The concept whereby protein binding to HS is not random but requires a limited number of sulfation patterns is becoming clear. Here we describe a hydrophobic trapping assay for screening a library of heparin hexasaccharides for binders to antithrombin III (ATIII). The hexasaccharide compositions are defined with their building block content in the following format: (DeltaHexA:HexA:GlcN:SO 3:Ac). Of five initial compositions present in the library, (1:2:3:6:1), (1:2:3:7:1), (1:2:3:7:0), (1:2:3:8:0), and (1:2:3:9:0), only two are shown to bind ATIII, namely, (1:2:3:8:0) and (1:2:3:9:0). The use of amide hydrophilic interaction (HILIC) liquid chromatography-mass spectrometry permitted reproducible quantitative analysis of the composition of the initial library as well as that of the binding fraction. The specificity of the hexasaccharides binding ATIII was confirmed by assaying their ability to enhance ATIII-mediated inhibition of Factor Xa in vitro.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.