ArticleThe Journal of clinical investigation2008
Mucosal IL-10 and TGF-beta play crucial roles in preventing LPS-driven, IFN-gamma-mediated epithelial damage in human colon explants.
Article in The Journal of clinical investigation, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 87 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
87 citing papers in PubMed, 148 citations in OpenAlex.
- Interleukin (IL)-23 suppresses IL-10 in inflammatory bowel disease.The Journal of biological chemistry · 2012Trial
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- The short-chain fatty acid butyrate prevents gut-brain amyloid-β pathology and neuroinflammation in an Alzheimer mouse model.Molecular psychiatry · 2026Article
- Alleviating the Adverse Effects of Lipopolysaccharide on Production Performance, Immune Function, and Intestinal Damage in Broilers via Dietary Alkaline Phosphatase.Animals : an open access journal from MDPI · 2026Article
- The role of IL-10 in antiviral immune memory development.ImmunoHorizons · 2026Review
- Gastrointestinal acute graft versus host disease: a translational perspective from pathogenesis to precision prevention and treatment.Frontiers in immunology · 2026Review
- Article
- Growth Performance, Immuno-Oxidant Status, Intestinal Health, Gene Expression, and Histomorphology of Growing Quails Fed Diets Supplemented with Essential Oils and Probiotics.Veterinary sciences · 2025Article
- Miniature chicken ileal explant culture to investigate the inflammatory response induced by pathogen-associated molecular patterns.Frontiers in veterinary science · 2025Article
- Early subclinical stages of the inflammatory bowel diseases: insights from human and animal studies.American journal of physiology. Gastrointestinal and liver physiology · 2025Review
- The perioperative management of geriatric patients in transplantation surgery-clinical, immunological, and translational considerations.Frontiers in transplantation · 2025Review
- Neisseria gonorrhoeae induces local secretion of IL-10 at the human cervix to promote colonization.The Journal of clinical investigation · 2024Article
- Article
- Immunomodulatory properties of chicken cathelicidin-2 investigated on an ileal explant culture.Veterinary research communications · 2024Article
- Mannanase improves the growth performance of broilers by alleviating inflammation of the intestinal epithelium and improving intestinal microbiota.Animal nutrition (Zhongguo xu mu shou yi xue hui) · 2024Article
- Sonic vibration ameliorates inflammatory diseases via the up-regulation of IL-10.Animal cells and systems · 2024Article
- Interaction between peripheral blood mononuclear cells andFrontiers in immunology · 2024Article
- In Situ Identification of Both IL-4 and IL-10 Cytokine-Receptor Interactions during Tissue Regeneration.Cells · 2023Article
- Canine Distemper Virus Alters Defense Responses in an Ex Vivo Model of Pulmonary Infection.Viruses · 2023Article
- Ammonia nitrogen stress damages the intestinal mucosal barrier of yellow catfish (Frontiers in physiology · 2023Article
27 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
IL-10 is an immunomodulatory cytokine that plays an obligate role in preventing spontaneous enterocolitis in mice. However, little is known about IL-10 function in the human intestinal mucosa. We showed here that IL-10 was constitutively expressed and secreted by the human normal colonic mucosa, including epithelial cells. Depletion of IL-10 in mucosal explants induced both downregulation of the IL-10-inducible, immunosuppressive gene BCL3 and upregulation of IFN-gamma, TNF-alpha, and IL-17. Interestingly, TGF-beta blockade also strongly induced IFN-gamma production. In addition, the high levels of IFN-gamma produced upon IL-10 depletion were responsible for surface epithelium damage and crypt loss, mainly by apoptosis. Polymyxin B, used as a scavenger of endogenous LPS, abolished both IFN-gamma production and epithelial barrier disruption. Finally, adding a commensal bacteria strain to mucosa explant cultures depleted of both IL-10 and LPS reproduced the ability of endogenous LPS to induce IFN-gamma secretion. These findings demonstrate that IL-10 ablation leads to an endogenous IFN-gamma-mediated inflammatory response via LPS from commensal bacteria in the human colonic mucosa. We also found that both IL-10 and TGF-beta play crucial roles in maintaining human colonic mucosa homeostasis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.