ArticleThe Journal of clinical investigation2008
PI3K signaling of autophagy is required for starvation tolerance and virulenceof Cryptococcus neoformans.
Article in The Journal of clinical investigation, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 70 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
70 citing papers in PubMed, 214 citations in OpenAlex.
- Multifunctional Roles of Autophagy in Fungi.Journal of fungi (Basel, Switzerland) · 2026Review
- Methionine suppresses autophagy in Cryptococcus neoformans: Impact of GPP2 gene deletion on the expression of autophagy-related genes.Folia microbiologica · 2026Article
- Evaluating the Antifungal Potential of Autophagy-Related Protein 4 (ATG4) Inhibitors against Human Fungal Pathogens.Journal of microbiology and biotechnology · 2025Article
- Roles of theJournal of fungi (Basel, Switzerland) · 2025Article
- United toward a common goal: Coordination of microbial virulence phenotypes.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Arg1 frommBio · 2024Article
- EIF4A3 Acts on the PI3K-AKT-ERK1/2-P70S6K Pathway through FLOT1 to Influence the Development of Lung Adenocarcinoma.Molecular cancer research : MCR · 2023Article
- Candidate genes potentially involved in molting and body size reduction in the male of the horned gall aphid,Frontiers in physiology · 2023Article
- Protein Kinase A Regulates Autophagy-Associated Proteins Impacting Growth and Virulence ofJournal of fungi (Basel, Switzerland) · 2022Article
- Class III PI3K Biology.Current topics in microbiology and immunology · 2022Article
- Article
- Role of the ESCRT Pathway in Laccase Trafficking and Virulence of Cryptococcus neoformans.Infection and immunity · 2020Article
- Autophagy Regulates Fungal Virulence and Sexual Reproduction inFrontiers in cell and developmental biology · 2020Article
- Biological functions of the autophagy-related proteins Atg4 and Atg8 in Cryptococcus neoformans.PloS one · 2020Article
- RNA-seq analysis provides insight into molecular adaptations of Andrias davidianus.Development genes and evolution · 2019Article
- Pathogenic Fungal Infection in the Lung.Frontiers in immunology · 2019Review
- Conserved Autophagy Pathway Contributes to Stress Tolerance and Virulence and Differentially Controls Autophagic Flux Upon Nutrient Starvation inFrontiers in microbiology · 2019Article
- Protein Kinases at the Intersection of Translation and Virulence.Frontiers in cellular and infection microbiology · 2019Review
- Article
- Immunology of Cryptococcal Infections: Developing a Rational Approach to Patient Therapy.Frontiers in immunology · 2018Review
10 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
12 authors at 2 institutions in 2 countries.
Funding
Abstract
Autophagy is a process by which cells recycle cytoplasm and defective organelles during stress situations such as nutrient starvation. It can also be used by host cells as an immune defense mechanism to eliminate infectious pathogens. Here we describe the use of autophagy as a survival mechanism and virulence-associated trait by the human fungal pathogen Cryptococcus neoformans. We report that a mutant form of C. neoformans lacking the Vps34 PI3K (vps34Delta), which is known to be involved in autophagy in ascomycete yeast, was defective in the formation of autophagy-related 8-labeled (Atg8-labeled) vesicles and showed a dramatic attenuation in virulence in mouse models of infection. In addition, autophagic vesicles were observed in WT but not vps34Delta cells after phagocytosis by a murine macrophage cell line, and Atg8 expression was exhibited in WT C. neoformans during human infection of brain. To dissect the contribution of defective autophagy in vps34Delta C. neoformans during pathogenesis, a strain of C. neoformans in which Atg8 expression was knocked down by RNA interference was constructed and these fungi also demonstrated markedly attenuated virulence in a mouse model of infection. These results demonstrated PI3K signaling and autophagy as a virulence-associated trait and survival mechanism during infection with a fungal pathogen. Moreover, the data show that molecular dissection of such pathogen stress-response pathways may identify new approaches for chemotherapeutic interventions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.