ArticleThe Journal of molecular diagnostics : JMD2008
Simultaneous isolation of DNA and RNA from the same cell population obtained by laser capture microdissection for genome and transcriptome profiling.
Article in The Journal of molecular diagnostics : JMD, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- A modified dual preparatory method for improved isolation of nucleic acids from laser microdissected fresh-frozen human cancer tissue specimens.Biology methods & protocols · 2024Article
- Genetic dissection of intratumor heterogeneity of PD-L1 expression in EGFR-mutated lung adenocarcinoma.Thoracic cancer · 2023Article
- Multi-omics HeCaToS dataset of repeated dose toxicity for cardiotoxic & hepatotoxic compounds.Scientific data · 2022Article
- A rapid and sensitive system for recovery of nucleic acids from Mycobacteria sp. on archived glass slides.BMC microbiology · 2018Article
- Comprehensive chromosome screening and gene expression analysis from the same biopsy in human preimplantation embryos.Molecular human reproduction · 2017Article
- A method for obtaining simian immunodeficiency virus RNA sequences from laser capture microdissected and immune captured CD68+ and CD163+ macrophages from frozen tissue sections of bone marrow and brain.Journal of immunological methods · 2017Article
- Coextraction and PCR Based Analysis of Nucleic Acids From Formalin-Fixed Paraffin-Embedded Specimens.Journal of clinical laboratory analysis · 2015Article
- Quantitative gene analysis of methylation and expression (Q-GAME) in fresh or fixed cells and tissues.Experimental dermatology · 2014Article
- Percutaneous image-guided biopsy of prostate cancer metastases yields samples suitable for genomics and personalised oncology.Clinical & experimental metastasis · 2014Article
- Differential Gene Expression Landscape of Co-Existing Cervical Pre-Cancer Lesions Using RNA-seq.Frontiers in oncology · 2014Article
- Tumor evolution and intratumor heterogeneity of an oropharyngeal squamous cell carcinoma revealed by whole-genome sequencing.Neoplasia (New York, N.Y.) · 2013Article
- Selective nucleic acid removal via exclusion (SNARE): capturing mRNA and DNA from a single sample.Analytical chemistry · 2013Article
- Optimizing Frozen Sample Preparation for Laser Microdissection: Assessment of CryoJane Tape-Transfer System®.PloS one · 2013Article
- Integrative genomics in combination with RNA interference identifies prognostic and functionally relevant gene targets for oral squamous cell carcinoma.PLoS genetics · 2013Article
- Sol-gel synthesis, characterization, and in vitro compatibility of iron nanoparticle-encapsulating silica microspheres for hyperthermia in cancer therapy.Journal of materials science. Materials in medicine · 2012Article
- Integrative analysis in oral squamous cell carcinoma reveals DNA copy number-associated miRNAs dysregulating target genes.Otolaryngology--head and neck surgery : official journal of American Academy of Otolaryngology-Head and Neck Surgery · 2012Article
- Single-cell and regional gene expression analysis in Alzheimer's disease.Cellular and molecular neurobiology · 2012Review
- Biospecimens and biorepositories: from afterthought to science.Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology · 2012Article
- Maintaining Breast Cancer Specimen Integrity and Individual or Simultaneous Extraction of Quality DNA, RNA, and Proteins from Allprotect-Stabilized and Nonstabilized Tissue Samples.Biopreservation and biobanking · 2011Article
- Simultaneous DNA and RNA isolation from brain punches for epigenetics.BMC research notes · 2011Article
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Authors and funding
11 authors.
Funding
Abstract
Laser capture microdissection (LCM) is used extensively for genome and transcriptome profiling. Traditionally, however, DNA and RNA are purified from separate populations of LCM-harvested cells, limiting the strength of inferences about the relationship between gene expression and gene sequence variation. There have been no published protocols for the simultaneous isolation of DNA and RNA from the same cells that are obtained by LCM of patient tissue specimens. Here we report an adaptation of the Qiagen AllPrep method that allows the purification of DNA and RNA from the same LCM-harvested cells. We compared DNA and RNA purified by the QIAamp DNA Micro kit and the PicoPure RNA Isolation kit, respectively, from LCM-collected cells from adjacent tissue sections of the same specimen. The adapted method yields 90% of DNA and 38% of RNA compared with the individual methods. When tested with the GeneChip 250K Nsp Array, the concordance rate of the single nucleotide polymorphism heterozygosity calls was 98%. When tested with the GeneChip U133 Plus 2.0 Array, the correlation coefficient of the raw gene expression was 97%. Thus, we developed a method to obtain both DNA and RNA material from a single population of LCM-harvested cells and herein discuss the strengths and limitations of this methodology.
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