ArticlePlant physiology2008
Characteristic and expression analysis of a metallothionein gene, OsMT2b, down-regulated by cytokinin suggests functions in root development and seed embryo germination of rice.
Article in Plant physiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 60 papers.
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60 citing papers in PubMed, 141 citations in OpenAlex.
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- Integrated metabolome and transcriptome profiling demonstrates dynamic regulatory roles of hormones in direct-seeding rice.Frontiers in plant science · 2026Article
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- QTL mapping and candidate gene analysis of element accumulation in rice grains via genome-wide association study and population genetic analysis.BMC plant biology · 2025Article
- OsAPSE modulates non-covalent interactions between arabinogalactan proteinFrontiers in plant science · 2025Article
- Metallothionein family genes in kiwifruit: characterization and determining their roles in plant's response to different stresses.Frontiers in plant science · 2024Article
- Characterization of the Metallothionein Gene Family inAntioxidants (Basel, Switzerland) · 2023Article
- Nutritional Genomic Approach for Improving Grain Protein Content in Wheat.Foods (Basel, Switzerland) · 2023Article
- Changes in physio-biochemical parameters and expression of metallothioneins in Avena sativa L. in response to drought.Scientific reports · 2023Article
- Genome-Wide Association Study Reveals Candidate Genes for Root-Related Traits in Rice.Current issues in molecular biology · 2022Article
- Isolation and Characterization of thePlants (Basel, Switzerland) · 2022Article
- Metallothionein1A Regulates Rhizobial Infection and Nodulation inInternational journal of molecular sciences · 2022Article
- The intertwining of Zn-finger motifs and abiotic stress tolerance in plants: Current status and future prospects.Frontiers in plant science · 2022Review
- Effects of Flooding and Endogenous Hormone on the Formation of Knee Roots inFrontiers in plant science · 2022Article
- Genome-Wide Identification and Analysis of the Metallothionein Genes inInternational journal of molecular sciences · 2021Article
- Proofing Direct-Seeded Rice with Better Root Plasticity and Architecture.International journal of molecular sciences · 2021Review
- Genome-Wide Association Study Reveals the QTLs for Seed Storability in World Rice Core Collections.Plants (Basel, Switzerland) · 2021Article
- High-resolution genome-wide association study pinpoints metal transporter and chelator genes involved in the genetic control of element levels in maize grain.G3 (Bethesda, Md.) · 2021Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Metallothioneins (MTs) are low molecular mass and cysteine-rich metal-binding proteins known to be mainly involved in maintaining metal homeostasis and stress responses. But, their functions in higher plant development are scarcely studied. Here, we characterized rice (Oryza sativa) METALLOTHIONEIN2b (OsMT2b) molecularly and found that its expression was down-regulated by cytokinins. OsMT2b was preferentially expressed in rice immature panicles, scutellum of germinating embryos, and primordium of lateral roots. In contrast with wild-type plants, OsMT2b-RNA interference (RNAi) transgenic plants had serious handicap in plant growth and root formation, whereas OsMT2b-overexpressing transformants were dwarfed and presented more adventitious roots and big lateral roots. The increased cytokinin levels in RNAi plants and decreased cytokinin levels in overexpressing plants were confirmed by high-performance liquid chromatography quantitative analysis in the roots of wild-type and transgenic plants. In RNAi plants, localization of isopentenyladenosine, a kind of endogenous cytokinin, in roots and germinating embryos expanded to the whole tissues, whereas in overexpressing plants, the isopentenyladenosine signals were very faint in the vascular tissues of roots and scutellum cells of germinating embryos. In vitro culture of embryos could largely resume the reduced germination frequency in RNAi plants but had no obvious change in overexpressing plants. Taken together, these results indicate a possible feedback regulation mechanism of OsMT2b to the level of endogenous cytokinins that is involved in root development and seed embryo germination of rice.
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