ArticleJournal of molecular biology2008
Mapping the cofilin binding site on yeast G-actin by chemical cross-linking.
Article in Journal of molecular biology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 38 citations in OpenAlex.
- Drebrin is Required for Myosin-facilitated Actin Cytoskeletal Remodeling during Pulmonary Alveolar Development.American journal of respiratory cell and molecular biology · 2024Article
- Actin Isoform Composition and Binding Factors Fine-Tune Regulatory Impact of Mical Enzymes.International journal of molecular sciences · 2023Article
- Magic angle spinning NMR structure of human cofilin-2 assembled on actin filaments reveals isoform-specific conformation and binding mode.Nature communications · 2022Article
- Structural Analysis of Human Cofilin 2/Filamentous Actin Assemblies: Atomic-Resolution Insights from Magic Angle Spinning NMR Spectroscopy.Scientific reports · 2017Article
- F-actin dismantling through a redox-driven synergy between Mical and cofilin.Nature cell biology · 2016Article
- Site-specific cation release drives actin filament severing by vertebrate cofilin.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Drebrin inhibits cofilin-induced severing of F-actin.Cytoskeleton (Hoboken, N.J.) · 2014Article
- A mechanism for actin filament severing by malaria parasite actin depolymerizing factor 1 via a low affinity binding interface.The Journal of biological chemistry · 2014Article
- Cofilin-induced changes in F-actin detected via cross-linking with benzophenone-4-maleimide.Biochemistry · 2013Article
- Drebrin-induced stabilization of actin filaments.The Journal of biological chemistry · 2013Article
- Identification of cation-binding sites on actin that drive polymerization and modulate bending stiffness.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Glutamyl phosphate is an activated intermediate in actin crosslinking by actin crosslinking domain (ACD) toxin.PloS one · 2012Article
- Mutant profilin suppresses mutant actin-dependent mitochondrial phenotype in Saccharomyces cerevisiae.The Journal of biological chemistry · 2011Article
- The interaction of cofilin with the actin filament.Journal of molecular biology · 2011Article
- Cofilin-linked changes in actin filament flexibility promote severing.Biophysical journal · 2011Article
- A nucleotide state-sensing region on actin.The Journal of biological chemistry · 2010Article
- Elucidating the higher-order structure of biopolymers by structural probing and mass spectrometry: MS3D.Journal of mass spectrometry : JMS · 2010Article
- Mapping of drebrin binding site on F-actin.Journal of molecular biology · 2010Article
- Antiparallel dimer and actin assembly.Biochemistry · 2010Article
- ADF/Cofilin-actin rods in neurodegenerative diseases.Current Alzheimer research · 2010Review
Corrections and comments
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Authors and funding
11 authors at 3 institutions in 2 countries.
Funding
Abstract
Cofilin is a major cytoskeletal protein that binds to both monomeric actin (G-actin) and polymeric actin (F-actin) and is involved in microfilament dynamics. Although an atomic structure of the G-actin-cofilin complex does not exist, models of the complex have been built using molecular dynamics simulations, structural homology considerations, and synchrotron radiolytic footprinting data. The hydrophobic cleft between actin subdomains 1 and 3 and, alternatively, the cleft between actin subdomains 1 and 2 have been proposed as possible high-affinity cofilin binding sites. In this study, the proposed binding of cofilin to the subdomain 1/subdomain 3 region on G-actin has been probed using site-directed mutagenesis, fluorescence labeling, and chemical cross-linking, with yeast actin mutants containing single reactive cysteines in the actin hydrophobic cleft and with cofilin mutants carrying reactive cysteines in the regions predicted to bind to G-actin. Mass spectrometry analysis of the cross-linked complex revealed that cysteine 345 in subdomain 1 of mutant G-actin was cross-linked to native cysteine 62 on cofilin. A cofilin mutant that carried a cysteine substitution in the alpha 3-helix (residue 95) formed a cross-link with residue 144 in actin subdomain 3. Distance constraints imposed by these cross-links provide experimental evidence for cofilin binding between actin subdomains 1 and 3 and fit a corresponding docking-based structure of the complex. The cross-linking of the N-terminal region of recombinant yeast cofilin to actin residues 346 and 374 with dithio-bis-maleimidoethane (12.4 A) and via disulfide bond formation was also documented. This set of cross-linking data confirms the important role of the N-terminal segment of cofilin in interactions with G-actin.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.