ArticleGene therapy2008
Optimization of a gene electrotransfer method for mesenchymal stem cell transfection.
Article in Gene therapy, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
26 citing papers in PubMed, 66 citations in OpenAlex.
- The Influence of Moderate Electroporation onMicroorganisms · 2025Article
- Cell-Penetrating Peptide-Mediated Biomolecule Transportation in Artificial Lipid Vesicles and Living Cells.Molecules (Basel, Switzerland) · 2024Review
- Research Progress on Cardiac Tissue Construction of Mesenchymal Stem Cells for Myocardial Infarction.Current stem cell research & therapy · 2024Review
- Effect of Experimental Electrical and Biological Parameters on Gene Transfer by Electroporation: A Systematic Review and Meta-Analysis.Pharmaceutics · 2022Review
- Genetically Engineered-MSC Therapies for Non-unions, Delayed Unions and Critical-size Bone Defects.International journal of molecular sciences · 2019Review
- Electroporation: A Sustainable and Cell Biology Preserving Cell Labeling Method for Adipogenous Mesenchymal Stem Cells.BioResearch open access · 2019Article
- Intranasal delivery of stem cell-based therapies for the treatment of brain malignancies.Expert opinion on drug delivery · 2018Review
- Targeted introduction and effective expression of hFIX at the AAVS1 locus in mesenchymal stem cells.Molecular medicine reports · 2017Article
- Demonstration of the Protein Involvement in Cell Electropermeabilization using Confocal Raman Microspectroscopy.Scientific reports · 2017Article
- Post-pulse addition of trans-cyclohexane-1,2-diol improves electrotransfer mediated gene expression in mammalian cells.Biochemistry and biophysics reports · 2016Article
- Impact of external medium conductivity on cell membrane electropermeabilization by microsecond and nanosecond electric pulses.Scientific reports · 2016Article
- Gene Electrotransfer of Canine Interleukin 12 into Canine Melanoma Cell Lines.The Journal of membrane biology · 2015Article
- A comparative study of non-viral gene delivery techniques to human adipose-derived mesenchymal stem cell.International journal of molecular sciences · 2014Article
- Different incubation times of cells after gene electrotransfer in fetal bovine serum affect cell viability, but not transfection efficiency.The Journal of membrane biology · 2014Article
- Cationic peptide exposure enhances pulsed-electric-field-mediated membrane disruption.PloS one · 2014Article
- Cyclodextrins in non-viral gene delivery.Biomaterials · 2014Review
- Robust, efficient, and practical electrogene transfer method for human mesenchymal stem cells using square electric pulses.Human gene therapy methods · 2013Article
- Electroporation for transfection and differentiation of dental pulp stem cells.BioResearch open access · 2013Article
- Nonviral gene targeting at rDNA locus of human mesenchymal stem cells.BioMed research international · 2013Article
- Development of an efficient, non-viral transfection method for studying gene function and bone growth in human primary cranial suture mesenchymal cells reveals that the cells respond to BMP2 and BMP3.BMC biotechnology · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Gene electrotransfer is an efficient and reproducible nonviral gene transfer technique useful for the nonpermanent expression of therapeutic transgenes. The present study established optimal conditions for the electrotransfer of reporter genes into mesenchymal stem cells (MSCs) isolated from rat bone marrow by their selective adherence to tissue-culture plasticware. The electrotransfer of the lacZ reporter gene was optimized by adjusting the pulse electric field intensity, electric pulse type, electropulsation buffer conductivity and electroporation temperature. LacZ electrotransfection into MSCs was optimal at 1500 V cm(-1) with pre-incubation in Spinner's minimum essential medium buffer at 22 degrees C. Under these conditions beta-galactosidase expression was achieved in 29+/-3% of adherent cells 48 h post transfection. The kinetics of beta-galactosidase activity revealed maintenance of beta-galactosidase production for at least 10 days. Moreover, electroporation did not affect the MSC potential for multidifferentiation; electroporated MSCs differentiated into osteoblastic, adipogenic and chondrogenic lineages to the same extent as cells that were not exposed to electric pulses. Thus, this study demonstrates the feasibility of efficient transgene electrotransfer into MSCs while preserving cell viability and multipotency.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.