ArticleThe EMBO journal2008
Cdk1 and Cdk2 activity levels determine the efficiency of replication origin firing in Xenopus.
Article in The EMBO journal, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 68 citations in OpenAlex.
- CDC7 and APC/CNature communications · 2026Article
- Adaptive Replication Fork Acceleration by CDK1-Cyclin B1 Sustains Genome Duplication despite Impaired Origin Firing.bioRxiv : the preprint server for biology · 2026Article
- A cyclin-dependent kinase-mediated phosphorylation switch of disordered protein condensation.Nature communications · 2023Article
- Review
- Review
- Review
- Organization of DNA Replication Origin Firing inGenes · 2021Article
- A predictable conserved DNA base composition signature defines human core DNA replication origins.Nature communications · 2020Article
- Regulation of the program of DNA replication by CDK: new findings and perspectives.Current genetics · 2019Review
- Stochastic Endogenous Replication Stress Causes ATR-Triggered Fluctuations in CDK2 Activity that Dynamically Adjust Global DNA Synthesis Rates.Cell systems · 2018Article
- CDK activity provides temporal and quantitative cues for organizing genome duplication.PLoS genetics · 2018Article
- Article
- Article
- Article
- Xenopus Cdc7 executes its essential function early in S phase and is counteracted by checkpoint-regulated protein phosphatase 1.Open biology · 2014Article
- DNA replication timing.Cold Spring Harbor perspectives in biology · 2013Review
- SUMO2/3 modification of cyclin E contributes to the control of replication origin firing.Nature communications · 2013Article
- Replication timing and its emergence from stochastic processes.Trends in genetics : TIG · 2012Review
- Regulation of the g1/s transition in hepatocytes: involvement of the cyclin-dependent kinase cdk1 in the DNA replication.International journal of hepatology · 2012Article
- The Ph1 locus suppresses Cdk2-type activity during premeiosis and meiosis in wheat.The Plant cell · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 5 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In this paper, we describe how, in a model embryonic system, cyclin-dependent kinase (Cdk) activity controls the efficiency of DNA replication by determining the frequency of origin activation. Using independent approaches of protein depletion and selective chemical inhibition of a single Cdk, we find that both Cdk1 and Cdk2 are necessary for efficient DNA replication in Xenopus egg extracts. Eliminating Cdk1, Cdk2 or their associated cyclins changes replication origin spacing, mainly by decreasing frequency of activation of origin clusters. Although there is no absolute requirement for a specific Cdk or cyclin, Cdk2 and cyclin E contribute more to origin cluster efficiency than Cdk1 and cyclin A. Relative Cdk activity required for DNA replication is very low, and even when both Cdk1 and Cdk2 are strongly inhibited, some origins are activated. However, at low levels, Cdk activity is limiting for the pre-replication complex to pre-initiation complex transition, origin activation and replication efficiency. As such, unlike mitosis, initiation of DNA replication responds progressively to changes in Cdk activity at low activity levels.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.