ArticleThe Journal of cell biology1991
Nucleotide specificities of anterograde and retrograde organelle transport in Reticulomyxa are indistinguishable.
Article in The Journal of cell biology, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 41 citations in OpenAlex.
- Constructing 3D microtubule networks using holographic optical trapping.Scientific reports · 2015Article
- Article
- High-throughput sequencing of Astrammina rara: sampling the giant genome of a giant foraminiferan protist.BMC genomics · 2011Article
- The JNM1 gene in the yeast Saccharomyces cerevisiae is required for nuclear migration and spindle orientation during the mitotic cell cycle.The Journal of cell biology · 1994Article
- A motility in the eukaryotic flagellum unrelated to flagellar beating.Proceedings of the National Academy of Sciences of the United States of America · 1993Article
- Physiological evidence for involvement of a kinesin-related protein during anaphase spindle elongation in diatom central spindles.The Journal of cell biology · 1992Article
- Myosin-I moves actin filaments on a phospholipid substrate: implications for membrane targeting.The Journal of cell biology · 1992Article
- Mitotic motors.The Journal of cell biology · 1991Review
- Nucleotide specificity of the enzymatic and motile activities of dynein, kinesin, and heavy meromyosin.The Journal of cell biology · 1991Article
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Authors and funding
4 authors at 1 institution in 1 country.
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Abstract
Membrane-bound organelles move bidirectionally along microtubules in the freshwater ameba, Reticulomyxa. We have examined the nucleotide requirements for transport in a lysed cell model and compared them with kinesin and dynein-driven motility in other systems. Both anterograde and retrograde transport in Reticulomyxa show features characteristic of dynein but not of kinesin-powered movements: organelle transport is reactivated only by ATP and no other nucleoside triphosphates; the Km and Vmax of the ATP-driven movements are similar to values obtained for dynein rather than kinesin-driven movement; and of 15 ATP analogues tested for their ability to promote organelle transport, only 4 of them did. This narrow specificity resembles that of dynein-mediated in vitro transport and is dissimilar to the broad specificity of the kinesin motor (Shimizu, T., K. Furusawa, S. Ohashi, Y. Y. Toyoshima, M. Okuno, F. Malik, and R. D. Vale. 1991. J. Cell Biol. 112: 1189-1197). Remarkably, anterograde and retrograde organelle transport cannot be distinguished at all with respect to nucleotide specificity, kinetics of movement, and the ability to use the ATP analogues. Since the "kinetic fingerprints" of the motors driving transport in opposite directions are indistinguishable, the same type of motor(s) may be involved in the two directions of movement.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.