ArticleRNA (New York, N.Y.)2008
Structure-function analysis of vaccinia virus mRNA cap (guanine-N7) methyltransferase.
Article in RNA (New York, N.Y.), 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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Who cites it
15 citing papers in PubMed.
- Structural basis for sensitivity and acquired resistance of fungal cap guanine-N7 methyltransferases to the antifungal antibiotic sinefungin.Nucleic acids research · 2025Article
- Characterization, Directed Evolution, and Targeting of DNA Virus-Encoded RNA Capping Enzymes Using Phenotypic Yeast Platforms.ACS chemical biology · 2023Article
- Versatile strategy using vaccinia virus-capping enzyme to synthesize functional 5' cap-modified mRNAs.Nucleic acids research · 2023Article
- The Role of RNA Modification in HIV-1 Infection.International journal of molecular sciences · 2022Review
- Structure and Biochemical Characteristic of the Methyltransferase (MTase) Domain of RNA Capping Enzyme from African Swine Fever Virus.Journal of virology · 2021Article
- Direct High-Throughput Screening Assay for mRNA Cap Guanine-N7 Methyltransferase Activity.Chemistry (Weinheim an der Bergstrasse, Germany) · 2020Article
- Antiviral effect of sinefungin on in vitro growth of feline herpesvirus type 1.The Journal of antibiotics · 2019Article
- Molecular basis of RNA guanine-7 methyltransferase (RNMT) activation by RAM.Nucleic acids research · 2016Article
- Crystal structure of vaccinia virus mRNA capping enzyme provides insights into the mechanism and evolution of the capping apparatus.Structure (London, England : 1993) · 2014Article
- Structure-function analysis of severe acute respiratory syndrome coronavirus RNA cap guanine-N7-methyltransferase.Journal of virology · 2013Article
- Orthopoxvirus targets for the development of new antiviral agents.Antiviral research · 2012Review
- Article
- Trimethylguanosine capping selectively promotes expression of Rev-dependent HIV-1 RNAs.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Characterization of a mimivirus RNA cap guanine-N2 methyltransferase.RNA (New York, N.Y.) · 2009Article
- Mutational analysis of vaccinia virus mRNA cap (guanine-N7) methyltransferase reveals essential contributions of the N-terminal peptide that closes over the active site.RNA (New York, N.Y.) · 2008Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
The guanine-N7 methyltransferase domain of vaccinia virus mRNA capping enzyme is a heterodimer composed of a catalytic subunit and a stimulatory subunit. Structure-function analysis of the catalytic subunit by alanine scanning and conservative substitutions (49 mutations at 25 amino acids) identified 12 functional groups essential for methyltransferase activity in vivo, most of which were essential for cap methylation in vitro. Defects in cap binding were demonstrated for a subset of lethal mutants that displayed residual activity in vitro. We discuss our findings in light of a model of the Michaelis complex derived from crystal structures of AdoHcy-bound vaccinia cap methyltransferase and GTP-bound cellular cap methyltransferase. The structure-function data yield a coherent picture of the vaccinia cap methyltransferase active site and the determinants of substrate specificity and affinity.
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