ArticleJournal of clinical microbiology2008
RespiFinder: a new multiparameter test to differentially identify fifteen respiratory viruses.
Article in Journal of clinical microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 66 papers, 2 of them syntheses that pooled it.
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Who cites it
66 citing papers in PubMed, 2 syntheses or guidelines pooled it.
- Multiplex PCR system for the rapid diagnosis of respiratory virus infection: systematic review and meta-analysis.Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases · 2018Pooled it
- Diagnostic Accuracy of Rapid Antigen Detection Tests for Respiratory Syncytial Virus Infection: Systematic Review and Meta-analysis.Journal of clinical microbiology · 2015Pooled it
- Performance evaluation of TaqMan Array Card real-time PCR for multi-pathogen detection in acute undifferentiated febrile illness.BMC infectious diseases · 2026Article
- Pathogen profiles in CSF from patients with acute infectious meningitis or encephalitis: a multicenter study based on multiplex PCR and mNGS in China, 2018-2019.BMC infectious diseases · 2025Article
- Dataset of acute respiratory infections: Epidemiological and etiological data from a cohort of 801 Moroccan children.Data in brief · 2024Article
- Design and Evaluation of Multiplex One-Step Reverse Transcription PCR-Dipstick Chromatography Method for the Analysis of Seven Respiratory Pathogens.Current microbiology · 2021Article
- Nosocomial Pneumonia in the Era of Multidrug-Resistance: Updates in Diagnosis and Management.Microorganisms · 2021Review
- SARS-CoV, MERS-CoV and SARS-CoV-2: A Diagnostic Challenge.Measurement : journal of the International Measurement Confederation · 2021Article
- Evaluation of TaqMan Array card (TAC) for the detection of 28 respiratory pathogens.BMC infectious diseases · 2020Article
- Human virus detection with graphene-based materials.Biosensors & bioelectronics · 2020Review
- Enteric Virome and Carcinogenesis in the Gut.Digestive diseases and sciences · 2020Review
- Method Comparison of the ImmuView L. pneumophila andJournal of clinical microbiology · 2020Article
- Molecular Diagnosis of Pneumonia Using Multiplex Real-Time PCR Assay RespiFinder® SMART 22 FAST in a Group of Moroccan Infants.Advances in virology · 2020Article
- Current and Future Point-of-Care Tests for Emerging and New Respiratory Viruses and Future Perspectives.Frontiers in cellular and infection microbiology · 2020Review
- Amplification chemistries in clinical virology.Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology · 2019Review
- Underweight, overweight, and obesity as independent risk factors for hospitalization in adults and children from influenza and other respiratory viruses.Influenza and other respiratory viruses · 2019Observational
- Diagnostic performance of multiplex PCR on pulmonary samples versus nasopharyngeal aspirates in community-acquired severe lower respiratory tract infections.Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology · 2018Article
- Laboratory Diagnosis of Respiratory Tract Infections in Children - the State of the Art.Frontiers in microbiology · 2018Review
- An Increased Frequency in HLA Class I Alleles and Haplotypes Suggests Genetic Susceptibility to Influenza A (H1N1) 2009 Pandemic: A Case-Control Study.Journal of immunology research · 2018Article
- Article
6 more citing papers are in PubMed but not listed here.
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Authors and funding
11 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Broad-spectrum analysis for pathogens in patients with respiratory tract infections is becoming more relevant as the number of potential infectious agents is still increasing. Here we describe the new multiparameter RespiFinder assay, which is based on the multiplex ligation-dependent probe amplification (MLPA) technology. This assay detects 15 respiratory viruses in one reaction. The MLPA reaction is preceded by a preamplification step which ensures the detection of both RNA and DNA viruses with the same specificity and sensitivity as individual monoplex real-time reverse transcription-PCRs. The RespiFinder assay was validated with 144 clinical samples, and the results of the assay were compared to those of cell culture and a respiratory syncytial virus (RSV)-specific immunochromatography assay (ICA). Compared to the cell culture results, the RespiFinder assay showed specificities and sensitivities of 98.2% and 100%, respectively, for adenovirus; 96.4% and 100%, respectively, for human metapneumovirus; 98.2% and 100%, respectively, for influenza A virus (InfA); 99.1% and 100%, respectively, for parainfluenza virus type 1 (PIV-1); 99.1% and 80%, respectively, for PIV-3; 90.1% and 100%, respectively, for rhinovirus; and 94.6% and 100%, respectively, for RSV. Compared to the results of the RSV-specific ICA, the RespiFinder assay gave a specificity and a sensitivity of 82.4% and 80%, respectively. PIV-2, PIV-4, influenza B virus, InfA H5N1, and coronavirus 229E were not detected in the clinical specimens tested. The use of the RespiFinder assay resulted in an increase in the diagnostic yield compared to that obtained by cell culture (diagnostic yields, 60% and 35.5%, respectively). In conclusion, the RespiFinder assay provides a user-friendly and high-throughput tool for the simultaneous detection of 15 respiratory viruses with excellent overall performance statistics.
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