ArticleThe Journal of biological chemistry2008
The Saccharomyces cerevisiae 60 S ribosome biogenesis factor Tif6p is regulated by Hrr25p-mediated phosphorylation.
Article in The Journal of biological chemistry, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 34 citations in OpenAlex.
- Eukaryote initiation factor 6 modulates small-cell lung carcinoma plasticity via the integrin-FAK signaling axis.Nature communications · 2026Article
- Mitochondrial Biogenesis Is Positively Regulated by Casein Kinase I Hrr25 Through Phosphorylation of Puf3 inGenetics · 2020Article
- It is all about the process(ing): P-body granules and the regulation of signal transduction.Current genetics · 2020Review
- Roles of Elongator Dependent tRNA Modification Pathways in Neurodegeneration and Cancer.Genes · 2018Review
- P-Body Localization of the Hrr25/Casein Kinase 1 Protein Kinase Is Required for the Completion of Meiosis.Molecular and cellular biology · 2018Article
- Molecular basis of the human ribosomopathy Shwachman-Diamond syndrome.Advances in biological regulation · 2018Review
- Wobble uridine modifications-a reason to live, a reason to die?!RNA biology · 2017Review
- Casein Kinase I Isoform Hrr25 Is a Negative Regulator of Haa1 in the Weak Acid Stress Response Pathway in Saccharomyces cerevisiae.Applied and environmental microbiology · 2017Article
- The Activity-Dependent Regulation of Protein Kinase Stability by the Localization to P-Bodies.Genetics · 2016Article
- Mechanism of eIF6 release from the nascent 60S ribosomal subunit.Nature structural & molecular biology · 2015Article
- Interaction of CK1δ with γTuSC ensures proper microtubule assembly and spindle positioning.Molecular biology of the cell · 2015Article
- Hrr25/CK1δ-directed release of Ltv1 from pre-40S ribosomes is necessary for ribosome assembly and cell growth.The Journal of cell biology · 2015Article
- Phosphorylation of Elp1 by Hrr25 is required for elongator-dependent tRNA modification in yeast.PLoS genetics · 2015Article
- Hrr25 kinase promotes selective autophagy by phosphorylating the cargo receptor Atg19.EMBO reports · 2014Article
- Translation initiation factor eIF3h targets specific transcripts to polysomes during embryogenesis.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Aspergillus nidulans CkiA is an essential casein kinase I required for delivery of amino acid transporters to the plasma membrane.Molecular microbiology · 2012Article
- Eukaryotic initiation factor 6, an evolutionarily conserved regulator of ribosome biogenesis and protein translation.Plant signaling & behavior · 2011Article
- Opposing action of casein kinase 1 and calcineurin in nucleo-cytoplasmic shuttling of mammalian translation initiation factor eIF6.The Journal of biological chemistry · 2011Article
- Yeast AEP3p is an accessory factor in initiation of mitochondrial translation.The Journal of biological chemistry · 2009Article
- Powering through ribosome assembly.RNA (New York, N.Y.) · 2009Review
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
The biosynthesis of 60 S ribosomal subunits in Saccharomyces cerevisiae requires Tif6p, the yeast homologue of mammalian eIF6. This protein is necessary for the formation of 60 S ribosomal subunits because it is essential for the processing of 35 S pre-rRNA to the mature 25 S and 5.8 S rRNAs. In the present work, using molecular genetic and biochemical analyses, we show that Hrr25p, an isoform of yeast casein kinase I, phosphorylates Tif6p both in vitro and in vivo. Tryptic phosphopeptide mapping of in vitro phosphorylated Tif6p by Hrr25p and (32)P-labeled Tif6p isolated from yeast cells followed by mass spectrometric analysis revealed that phosphorylation occurred on a single tryptic peptide at Ser-174. Sucrose gradient fractionation and coimmunoprecipitation experiments demonstrate that a small but significant fraction of Hrr25p is bound to 66 S preribosomal particles that also contain bound Tif6p. Depletion of Hrr25p from a conditional yeast mutant that fails to phosphorylate Tif6p was unable to process pre-rRNAs efficiently, resulting in significant reduction in the formation of 25 S rRNA. These results along with our previous observations that phosphorylatable Ser-174 is required for yeast cell growth and viability, suggest that Hrr25p-mediated phosphorylation of Tif6p plays a critical role in the biogenesis of 60 S ribosomal subunits in yeast cells.
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