ArticlePloS one2008
In Drosophila melanogaster the COM locus directs the somatic silencing of two retrotransposons through both Piwi-dependent and -independent pathways.
Article in PloS one, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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36 citing papers in PubMed, 55 citations in OpenAlex.
- Article
- A maternally programmed intergenerational mechanism enables male offspring to make piRNAs from Y-linked precursor RNAs in Drosophila.Nature cell biology · 2023Article
- Reactivation of a somatic errantivirus and germline invasion in Drosophila ovaries.Nature communications · 2023Article
- Targeting miRNAs and Other Non-Coding RNAs as a Therapeutic Approach: An Update.Non-coding RNA · 2023Review
- Article
- Review
- Special vulnerability of somatic niche cells to transposable element activation in Drosophila larval ovaries.Scientific reports · 2020Article
- Trapping a somatic endogenous retrovirus into a germline piRNA cluster immunizes the germline against further invasion.Genome biology · 2019Article
- Transcriptionally promiscuous "blurry" promoters in Tc1/Mobile DNA · 2019Article
- Protecting and Diversifying the Germline.Genetics · 2018Review
- Transposable element dynamics and PIWI regulation impacts lncRNA and gene expression diversity in Drosophila ovarian cell cultures.Genome research · 2014Article
- Untangling the web: the diverse functions of the PIWI/piRNA pathway.Molecular reproduction and development · 2013Review
- Article
- Article
- NucBase, an easy to use read mapper for small RNAs.Mobile DNA · 2013Article
- Mutation in a primate-conserved retrotransposon reveals a noncoding RNA as a mediator of infantile encephalopathy.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Homology-dependent silencing by an exogenous sequence in the Drosophila germline.G3 (Bethesda, Md.) · 2012Article
- Polycomb group-dependent, heterochromatin protein 1-independent, chromatin structures silence retrotransposons in somatic tissues outside ovaries.DNA research : an international journal for rapid publication of reports on genes and genomes · 2011Article
- Heterotypic piRNA Ping-Pong requires qin, a protein with both E3 ligase and Tudor domains.Molecular cell · 2011Article
- The Cutoff protein regulates piRNA cluster expression and piRNA production in the Drosophila germline.The EMBO journal · 2011Article
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Authors and funding
5 authors at 5 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundIn the Drosophila germ line, repeat-associated small interfering RNAs (rasiRNAs) ensure genomic stability by silencing endogenous transposable elements. This RNA silencing involves small RNAs of 26-30 nucleotides that are mainly produced from the antisense strand and function through the Piwi protein. Piwi belongs to the subclass of the Argonaute family of RNA interference effector proteins, which are expressed in the germline and in surrounding somatic tissues of the reproductive apparatus. In addition to this germ-line expression, Piwi has also been implicated in diverse functions in somatic cells. PRINCIPAL
findingsHere, we show that two LTR retrotransposons from Drosophila melanogaster, ZAM and Idefix, are silenced by an RNA silencing pathway that has characteristics of the rasiRNA pathway and that specifically recognizes and destroys the sense-strand RNAs of the retrotransposons. This silencing depends on Piwi in the follicle cells surrounding the oocyte. Interestingly, this silencing is active in all the somatic tissues examined from embryos to adult flies. In these somatic cells, while the silencing still involves the strict recognition of sense-strand transcripts, it displays the marked difference of being independent of the Piwi protein. Finally, we present evidence that in all the tissues examined, the repression is controlled by the heterochromatic COM locus.
conclusionOur data shed further light on the silencing mechanism that acts to target Drosophila LTR retrotransposons in somatic cells throughout fly development. They demonstrate that different RNA silencing pathways are involved in ovarian versus other somatic tissues, since Piwi is necessary for silencing in the former tissues but is dispensable in the latter. They further demonstrate that these pathways are controlled by the heterochromatic COM locus which ensures the overall protection of Drosophila against the detrimental effects of random retrotransposon mobilization.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.