ArticleInternational journal of plant genomics2007
Evaluation of global RNA amplification and its use for high-throughput transcript analysis of laser-microdissected endosperm.
Article in International journal of plant genomics, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- A Single Cell but Many Different Transcripts: A Journey into the World of Long Non-Coding RNAs.International journal of molecular sciences · 2020Review
- Genetic Material Manipulation and Modification by Optical Trapping and Nanosurgery-A Perspective.Frontiers in bioengineering and biotechnology · 2020Review
- A rapid preparation procedure for laser microdissection-mediated harvest of plant tissues for gene expression analysis.Plant methods · 2019Article
- Transcriptional profiling of cork oak phellogenic cells isolated by laser microdissection.Planta · 2018Article
- Experimental Design for Laser Microdissection RNA-Seq: Lessons from an Analysis of Maize Leaf Development.Journal of visualized experiments : JoVE · 2017Article
- Genomic dissection of the seed.Frontiers in plant science · 2014Review
- Article
- Molecular profiling of stomatal meristemoids reveals new component of asymmetric cell division and commonalities among stem cell populations in Arabidopsis.The Plant cell · 2011Article
- A novel procedure for the quantitative analysis of metabolites, storage products and transcripts of laser microdissected seed tissues of Brassica napus.Plant methods · 2011Article
- Statistical evaluation of transcriptomic data generated using the Affymetrix one-cycle, two-cycle and IVT-Express RNA labelling protocols with the Arabidopsis ATH1 microarray.Plant methods · 2010Article
- The syncytium-specific expression of the Orysa;KRP3 CDK inhibitor: implication of its involvement in the cell cycle control in the rice (Oryza sativa L.) syncytial endosperm.Journal of experimental botany · 2010Article
- Identification of cytoskeleton-associated genes expressed during Arabidopsis syncytial endosperm development.Plant signaling & behavior · 2009Article
- Isolation of RNA from laser-capture-microdissected giant cells at early differentiation stages suitable for differential transcriptome analysis.Molecular plant pathology · 2009Article
- A roadmap for zinc trafficking in the developing barley grain based on laser capture microdissection and gene expression profiling.Journal of experimental botany · 2009Article
- Transcriptome analysis of proliferating Arabidopsis endosperm reveals biological implications for the control of syncytial division, cytokinin signaling, and gene expression regulation.Plant physiology · 2008Article
- Gene expression analysis of interferon kappa in laser capture microdissected cervical epithelium.Analytical biochemistry · 2008Article
- Identification of genes associated with regenerative success of Xenopus laevis hindlimbs.BMC developmental biology · 2008Article
- Stamen abscission zone transcriptome profiling reveals new candidates for abscission control: enhanced retention of floral organs in transgenic plants overexpressing Arabidopsis ZINC FINGER PROTEIN2.Plant physiology · 2008Article
- Article
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3 authors.
Funding
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Abstract
Laser microdissection (LM) provides a useful method for isolating specific cells or tissues from biological samples. Here, we adapted microdissection protocols to allow high-resolution transcript analysis of different tissues from developing Arabidopsis seed. Sufficient RNA ( approximately 50 ng) was extracted from endosperm tissue for RT-PCR. However, to obtain enough RNA for microarray analyses, it was necessary to amplify the RNA. PCR- and IVT-based amplification methods were investigated and several important technical aspects of amplification were identified (such as target truncation and alterations in signal intensity). We found that when starting from only 50 ng of RNA, amplification methods based on PCR and IVT produced sufficient product for reliable microarray hybridizations, with two-round IVT giving the best results. Microarray analyses, using endosperm-derived RNA amplified by two-round IVT, reproducibly identified endosperm enriched marker genes. Thus, when combined with RNA-amplification protocols, LM is a robust and reliable technique for high-throughput tissue-specific gene expression analysis.
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