ArticleProceedings of the National Academy of Sciences of the United States of America2008
Assessing the potential of mutational strategies to elicit new phenotypes in industrial strains.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed.
- Adaptive cargo deletion as an evolutionary dead-end during phage-based directed evolution.BMC research notes · 2026Article
- Strategies to increase the robustness of microbial cell factories.Advanced biotechnology · 2024Review
- Improving acid resistance ofFrontiers in bioengineering and biotechnology · 2023Article
- Engineering prokaryotic regulator IrrE to enhance stress tolerance in budding yeast.Biotechnology for biofuels · 2020Article
- Article
- Systematic and synthetic approaches to rewire regulatory networks.Current opinion in systems biology · 2018Article
- Engineering global transcription to tune lipophilic properties inBiotechnology for biofuels · 2018Article
- Mutator genomes decay, despite sustained fitness gains, in a long-term experiment with bacteria.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Crz1p Regulates pH Homeostasis in Candida glabrata by Altering Membrane Lipid Composition.Applied and environmental microbiology · 2016Article
- Using global transcription machinery engineering (gTME) to improve ethanol tolerance of Zymomonas mobilis.Microbial cell factories · 2016Article
- DNA microarray of global transcription factor mutant reveals membrane-related proteins involved in n-butanol tolerance in Escherichia coli.Biotechnology for biofuels · 2016Article
- Origins of Cell-to-Cell Bioprocessing Diversity and Implications of the Extracellular Environment Revealed at the Single-Cell Level.Scientific reports · 2015Article
- Rapid prototyping of microbial cell factories via genome-scale engineering.Biotechnology advances · 2015Review
- Significantly improved solvent tolerance of Escherichia coli by global transcription machinery engineering.Microbial cell factories · 2015Article
- Rational selection and engineering of exogenous principal sigma factor (σ(HrdB)) to increase teicoplanin production in an industrial strain of Actinoplanes teichomyceticus.Microbial cell factories · 2014Article
- Bacterial sigma factors as targets for engineered or synthetic transcriptional control.Frontiers in bioengineering and biotechnology · 2014Review
- Strategies for the multiplex mapping of genes to traits.Microbial cell factories · 2013Review
- Using the Cre-lox system to randomize target gene expression states and generate diverse phenotypes.Biotechnology and bioengineering · 2013Article
- Protein engineering for metabolic engineering: current and next-generation tools.Biotechnology journal · 2013Review
- "Direct cloning in Lactobacillus plantarum: electroporation with non-methylated plasmid DNA enhances transformation efficiency and makes shuttle vectors obsolete".Microbial cell factories · 2012Article
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2 authors.
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Abstract
Industrial strains have been traditionally improved by rational approaches and combinatorial methods involving mutagenesis and selection. Recently, other methods have emerged, such as the use of artificial transcription factors and engineering of the native ones. As methods for generating genetic diversity continue to proliferate, the need for quantifying phenotypic diversity and, hence, assessing the potential of various genetic libraries for strain improvement becomes more pronounced. Here, we present a metric based on the quantification of phenotypic diversity, using Lactobacillus plantarum as a model organism. We found that phenotypic diversity can be introduced by mutagenesis of the principal sigma factor, that this diversity can be modulated by tuning the sequence diversity, and that this method compares favorably with commonly used protocols for chemical mutagenesis. The results of the diversity metric here developed also correlated well with the probability of finding improved mutants in the different libraries, as determined by recursive screening under stress. In addition, we subjected our libraries to lactic and inorganic acids and found strains with improved growth in both conditions, with a concomitant increase in lactate productivity.
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