ArticleThe Biochemical journal2008
Energization-dependent endogenous activation of proton conductance in skeletal muscle mitochondria.
Article in The Biochemical journal, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 73 citations in OpenAlex.
- Differences in Mitochondrial Coupling Reveal a Novel Signature of Mitohormesis in Muscle of Healthy Individuals.The Journal of clinical endocrinology and metabolism · 2016Trial
- Nucleoside diphosphate kinase strongly promotes GDP and ADP metabolism in the cell and affects endogenous proton leak in mitochondria - the kinase is hampered by oxidative phosphorylation inhibitors.Journal of enzyme inhibition and medicinal chemistry · 2025Article
- Mitochondrial uncoupling, energy substrate utilization, and brown adipose tissue as therapeutic targets in cancer.npj metabolic health and disease · 2025Review
- Direct and indirect targets of carboxyatractyloside, including overlooked toxicity toward nucleoside diphosphate kinase (NDPK) and mitochondrial HPharmaceutical biology · 2023Review
- Mitochondrial Respiration-Dependent ANT2-UCP2 Interaction.Frontiers in physiology · 2022Article
- Mitochondrial ion channels in pancreatic β-cells: Novel pharmacological targets for the treatment of Type 2 diabetes.British journal of pharmacology · 2021Review
- Nutritional Ketosis and Mitohormesis: Potential Implications for Mitochondrial Function and Human Health.Journal of nutrition and metabolism · 2018Review
- Uncoupling protein 1 controls reactive oxygen species in brown adipose tissue.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Direct real-time quantification of mitochondrial oxidative phosphorylation efficiency in permeabilized skeletal muscle myofibers.American journal of physiology. Cell physiology · 2016Article
- Skeletal muscle dysfunction is associated with derangements in mitochondrial bioenergetics (but not UCP3) in a rodent model of sepsis.American journal of physiology. Endocrinology and metabolism · 2015Article
- The effect of high-fat--high-fructose diet on skeletal muscle mitochondrial energetics in adult rats.European journal of nutrition · 2015Article
- Mitochondrial oxidative metabolism and uncoupling proteins in the failing heart.Heart failure reviews · 2015Review
- Effects of standardized extract of Ginkgo biloba leaves EGb761 on mitochondrial functions: mechanism(s) of action and dependence on the source of mitochondria and respiratory substrate.Journal of bioenergetics and biomembranes · 2014Article
- Different effects of guanine nucleotides (GDP and GTP) on protein-mediated mitochondrial proton leak.PloS one · 2014Article
- Coenzyme Q10 depletion in medical and neuropsychiatric disorders: potential repercussions and therapeutic implications.Molecular neurobiology · 2013Review
- Glutathionylation state of uncoupling protein-2 and the control of glucose-stimulated insulin secretion.The Journal of biological chemistry · 2012Article
- Fatty acids change the conformation of uncoupling protein 1 (UCP1).The Journal of biological chemistry · 2012Article
- Hydroxynonenal, a lipid peroxidation end product, stimulates uncoupling protein activity in Acanthamoeba castellanii mitochondria; the sensitivity of the inducible activity to purine nucleotides depends on the membranous ubiquinone redox state.Journal of bioenergetics and biomembranes · 2012Article
- Characteristics of the turnover of uncoupling protein 3 by the ubiquitin proteasome system in isolated mitochondria.Biochimica et biophysica acta · 2011Article
- Endurance exercise training blunts the deleterious effect of high-fat feeding on whole body efficiency.American journal of physiology. Regulatory, integrative and comparative physiology · 2011Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
Leak of protons into the mitochondrial matrix during substrate oxidation partially uncouples electron transport from phosphorylation of ADP, but the functions and source of basal and inducible proton leak in vivo remain controversial. In the present study we describe an endogenous activation of proton conductance in mitochondria isolated from rat and mouse skeletal muscle following addition of respiratory substrate. This endogenous activation increased with time, required a high membrane potential and was diminished by high concentrations of serum albumin. Inhibition of this endogenous activation by GDP [classically considered specific for UCPs (uncoupling proteins)], carboxyatractylate and bongkrekate (considered specific for the adenine nucleotide translocase) was examined in skeletal muscle mitochondria from wild-type and Ucp3-knockout mice. Proton conductance through endogenously activated UCP3 was calculated as the difference in leak between mitochondria from wild-type and Ucp3-knockout mice, and was found to be inhibited by carboxyatractylate and bongkrekate, but not GDP. Proton conductance in mitochondria from Ucp3-knockout mice was strongly inhibited by carboxyatractylate, bongkrekate and partially by GDP. We conclude the following: (i) at high protonmotive force, an endogenously generated activator stimulates proton conductance catalysed partly by UCP3 and partly by the adenine nucleotide translocase; (ii) GDP is not a specific inhibitor of UCP3, but also inhibits proton translocation by the adenine nucleotide translocase; and (iii) the inhibition of UCP3 by carboxyatractylate and bongkrekate is likely to be indirect, acting through the adenine nucleotide translocase.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.