ArticleMolecular and cellular biology2008
Artificial recruitment of mediator by the DNA-binding domain of Adr1 overcomes glucose repression of ADH2 expression.
Article in Molecular and cellular biology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 17 citations in OpenAlex.
- Revisiting the model for coactivator recruitment: Med15 can select its target sites independent of promoter-bound transcription factors.Nucleic acids research · 2024Article
- The yeast 14-3-3 proteins Bmh1 and Bmh2 regulate key signaling pathways.Frontiers in molecular biosciences · 2024Review
- Engineering eukaryote-like regulatory circuits to expand artificial control mechanisms for metabolic engineering in Saccharomyces cerevisiae.Communications biology · 2022Article
- Snf1-Dependent Transcription Confers Glucose-Induced Decay upon the mRNA Product.Molecular and cellular biology · 2016Article
- Beta Cell Formation in vivo Through Cellular Networking, Integration and Processing (CNIP) in Wild Type Adult Mice.Current pharmaceutical biotechnology · 2016Article
- Effects of glucose, ethanol and acetic acid on regulation of ADH2 gene from Lachancea fermentati.PeerJ · 2016Article
- Yeast 14-3-3 protein functions as a comodulator of transcription by inhibiting coactivator functions.The Journal of biological chemistry · 2014Article
- The metabolism beyond programmed cell death in yeast.Experimental cell research · 2012Review
- Transcriptional regulation of nonfermentable carbon utilization in budding yeast.FEMS yeast research · 2010Review
- Article
- The transcriptional coactivators SAGA, SWI/SNF, and mediator make distinct contributions to activation of glucose-repressed genes.The Journal of biological chemistry · 2008Article
- Promoter binding by the Adr1 transcriptional activator may be regulated by phosphorylation in the DNA-binding region.PloS one · 2008Article
- The Mediator complex and transcription regulation.Critical reviews in biochemistry and molecular biologyReview
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
The transcription factor Adr1 activates numerous genes in nonfermentable carbon source metabolism. An unknown mechanism prevents Adr1 from stably binding to the promoters of these genes in glucose-grown cells. Glucose depletion leads to Snf1-dependent binding. Chromatin immunoprecipitation showed that the Adr1 DNA-binding domain could not be detected at the ADH2 promoter under conditions in which the binding of the full-length protein occurred. This suggested that an activation domain is required for stable binding, and coactivators may stabilize the interaction with the promoter. Artificial recruitment of Mediator tail subunits by fusion to the Adr1 DNA-binding domain overcame both the inhibition of promoter binding and glucose repression of ADH2 expression. In contrast, an Adr1 DNA-binding domain-Tbp fusion did not overcome glucose repression, although it was an efficient activator of ADH2 expression under derepressing conditions. When Mediator was artificially recruited, ADH2 expression was independent of SNF1, SAGA, and Swi/Snf, whereas ADH2 expression was dependent on these factors with wild-type Adr1. These results suggest that in the presence of glucose, the ADH2 promoter is accessible to Adr1 but that other interactions that occur when glucose is depleted do not take place. Artificial recruitment of Mediator appears to overcome this requirement and to allow stable binding and transcription under normally inhibitory conditions.
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