ArticleCell division2008
Genetic analysis of the spindle checkpoint genes san-1, mdf-2, bub-3 and the CENP-F homologues hcp-1 and hcp-2 in Caenorhabditis elegans.
Article in Cell division, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 31 citations in OpenAlex.
- Induction and regulation of reversible suspended animation in C. elegans.Nature communications · 2026Article
- Induction and regulation of a reversible form of suspended animation inbioRxiv : the preprint server for biology · 2026Article
- Loss of the puromycin-sensitive aminopeptidase, PAM-1, triggers the spindle assembly checkpoint during the first mitotic division inmicroPublication biology · 2024Article
- Functional midbody assembly in the absence of a central spindle.The Journal of cell biology · 2022Article
- Protection of the C. elegans germ cell genome depends on diverse DNA repair pathways during normal proliferation.PloS one · 2021Article
- Kinetochore Recruitment of the Spindle and Kinetochore-Associated (Ska) Complex Is Regulated by Centrosomal PP2A inGenetics · 2019Article
- Article
- Centromere protein F includes two sites that couple efficiently to depolymerizing microtubules.The Journal of cell biology · 2015Article
- Suspended animation, diapause and quiescence: arresting the cell cycle in C. elegans.Cell cycle (Georgetown, Tex.) · 2012Review
- Studying synthetic lethal interactions in the zebrafish system: insight into disease genes and mechanisms.Disease models & mechanisms · 2012Review
- NPP-16/Nup50 function and CDK-1 inactivation are associated with anoxia-induced prophase arrest in Caenorhabditis elegans.Molecular biology of the cell · 2010Article
- Spermatogenesis-specific features of the meiotic program in Caenorhabditis elegans.PLoS genetics · 2009Article
- Article
- Systematic analysis in Caenorhabditis elegans reveals that the spindle checkpoint is composed of two largely independent branches.Molecular biology of the cell · 2009Article
- The spindle assembly checkpoint in Caenorhabditis elegans: one who lacks Mad1 becomes mad one.Cell cycle (Georgetown, Tex.) · 2009Article
- Key players in chromosome segregation in Caenorhabditis elegans.Frontiers in bioscience (Landmark edition) · 2009Review
- SPDL-1 functions as a kinetochore receptor for MDF-1 in Caenorhabditis elegans.The Journal of cell biology · 2008Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundThe spindle checkpoint delays the onset of anaphase until all sister chromatids are aligned properly at the metaphase plate. To investigate the role san-1, the MAD3 homologue, has in Caenorhabditis elegans embryos we used RNA interference (RNAi) to identify genes synthetic lethal with the viable san-1(ok1580) deletion mutant.
resultsThe san-1(ok1580) animal has low penetrating phenotypes including an increased incidence of males, larvae arrest, slow growth, protruding vulva, and defects in vulva morphogenesis. We found that the viability of san-1(ok1580) embryos is significantly reduced when HCP-1 (CENP-F homologue), MDF-1 (MAD-1 homologue), MDF-2 (MAD-2 homologue) or BUB-3 (predicted BUB-3 homologue) are reduced by RNAi. Interestingly, the viability of san-1(ok1580) embryos is not significantly reduced when the paralog of HCP-1, HCP-2, is reduced. The phenotype of san-1(ok1580);hcp-1(RNAi) embryos includes embryonic and larval lethality, abnormal organ development, and an increase in abnormal chromosome segregation (aberrant mitotic nuclei, anaphase bridging). Several of the san-1(ok1580);hcp-1(RNAi) animals displayed abnormal kinetochore (detected by MPM-2) and microtubule structure. The survival of mdf-2(RNAi);hcp-1(RNAi) embryos but not bub-3(RNAi);hcp-1(RNAi) embryos was also compromised. Finally, we found that san-1(ok1580) and bub-3(RNAi), but not hcp-1(RNAi) embryos, were sensitive to anoxia, suggesting that like SAN-1, BUB-3 has a functional role as a spindle checkpoint protein.
conclusionTogether, these data suggest that in the C. elegans embryo, HCP-1 interacts with a subset of the spindle checkpoint pathway. Furthermore, the fact that san-1(ok1580);hcp-1(RNAi) animals had a severe viability defect whereas in the san-1(ok1580);hcp-2(RNAi) and san-1(ok1580);hcp-2(ok1757) animals the viability defect was not as severe suggesting that hcp-1 and hcp-2 are not completely redundant.
Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.