ArticleMolecular vision2008
Transplantation of a sheet of human corneal endothelial cell in a rabbit model.
Article in Molecular vision, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 47 citations in OpenAlex.
- Restorative and regenerative therapies for vision loss.Eye (London, England) · 2026Review
- Near infra-red labelling and tracking of corneal endothelial cells in-vivo.Scientific reports · 2022Article
- 3D in vitro model for human corneal endothelial cell maturation.Experimental eye research · 2019Article
- In Vivo Functionality of a Corneal Endothelium Transplanted by Cell-Injection Therapy in a Feline Model.Investigative ophthalmology & visual science · 2016Article
- Substrates for Expansion of Corneal Endothelial Cells towards Bioengineering of Human Corneal Endothelium.Journal of functional biomaterials · 2015Review
- N-isopropylacrylamide-co-glycidylmethacrylate as a thermoresponsive substrate for corneal endothelial cell sheet engineering.BioMed research international · 2014Article
- Regulation of functional corneal endothelial cells isolated from sphere colonies by Rho-associated protein kinase inhibitor.Experimental and therapeutic medicine · 2013Article
- Transplantation of tissue-engineered human corneal endothelium in cat models.Molecular vision · 2013Article
- Graft survival and endothelial outcomes in the new era of endothelial keratoplasty.Experimental eye research · 2012Review
- In vitro culture of human fetal corneal endothelial cells.Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie · 2011Article
- Establishment of a continuous untransfected human corneal endothelial cell line and its biocompatibility to denuded amniotic membrane.Molecular vision · 2011Article
- Reconstruction of a human cornea by the self-assembly approach of tissue engineering using the three native cell types.Molecular vision · 2010Article
- Fuchs' corneal dystrophy.Expert review of ophthalmology · 2010Article
- Establishment and characterization of a novel untransfected corneal endothelial cell line from New Zealand white rabbits.Molecular vision · 2009Article
- Human corneal endothelial cell transplantation in a human ex vivo model.Investigative ophthalmology & visual science · 2009Article
- Differential protein expression in human corneal endothelial cells cultured from young and older donors.Molecular vision · 2008Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeTo develop a novel method for constructing a sheet of human corneal endothelial cells (HCECs) and examine the properties of the HCEC sheet.
methodsHCECs were cultured on a cell culture insert for a week; ethylenediamine tetraacetic acid was applied from the bottom of the cell culture insert to attenuate the attachment of HCECs. The sheet of HCECs was constructed by bluntly detaching the cell sheet with a spatula. HCEC cell sheets were placed on the posterior surface of excised rabbit corneal buttons and transplanted onto the corneal beds of donor rabbits. In two eyes from the HCEC sheet group, cultured HCECs were labeled with PKH26 to observe the localization of HCECs after transplantation.
resultsCultured HCECs could be bluntly detached en bloc from the bottom of a culture insert. Immunostaining for ZO-1, Na+, K+-ATPase, laminin, fibronectin, and type IV collagen was positive in the cell sheet. The average cell density in a HCEC sheet was 2,425 cells/mm(2). After HCEC sheet transplantation, corneal edema decreased much earlier in the HCEC group than in the control group. In the HCEC sheet group, the monolayer of continuous cells attached to the posterior surface of the transplanted rabbit cornea and the posterior surface of transplanted cornea was covered with PKH26-labeled cells. The average endothelial cell density in the HCEC sheet group seven days postoperatively was 2,244 cells/mm(2).
conclusionsThis technique for producing an HCEC sheet might be useful in regenerative medicine for the cornea and reconstruction of the corneal endothelium.
Indexed as
Identifiers
18246029PMC2267690W2121438608What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.