ArticleMolecular vision2008
Effect of immunomodulation with anti-CD40L antibody on adenoviral-mediated transgene expression in mouse anterior segment.
Article in Molecular vision, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 34 citations in OpenAlex.
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- The Inducible TGFβ2 Ocular Hypertension Mouse Model.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Viral Vector-Induced Ocular Hypertension in Mice.Methods in molecular biology (Clifton, N.J.) · 2023Article
- A Novel Mouse Model of TGFβ2-Induced Ocular Hypertension Using Lentiviral Gene Delivery.International journal of molecular sciences · 2022Article
- Age and sex affect TGFβ2-induced ocular hypertension in C57BL/6J mice.Experimental eye research · 2022Article
- Using CRISPR Interference as a Therapeutic Approach to Treat TGFβ2-Induced Ocular Hypertension and Glaucoma.Investigative ophthalmology & visual science · 2021Article
- Impaired TRPV4-eNOS signaling in trabecular meshwork elevates intraocular pressure in glaucoma.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Autophagy stimulation reduces ocular hypertension in a murine glaucoma model via autophagic degradation of mutant myocilin.JCI insight · 2021Article
- Recombinant adenovirus causes prolonged mobilization of macrophages in the anterior chambers of mice.Molecular vision · 2021Article
- Inducible rodent models of glaucoma.Progress in retinal and eye research · 2020Review
- The Role of Wnt/β-Catenin Signaling and K-Cadherin in the Regulation of Intraocular Pressure.Investigative ophthalmology & visual science · 2018Article
- CRISPR-Cas9-based treatment of myocilin-associated glaucoma.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Gene transfer to the outflow tract.Experimental eye research · 2017Review
- Elevation of intraocular pressure in rodents using viral vectors targeting the trabecular meshwork.Experimental eye research · 2015Review
- Gremlin Induces Ocular Hypertension in Mice Through Smad3-Dependent Signaling.Investigative ophthalmology & visual science · 2015Article
- Smad3 is necessary for transforming growth factor-beta2 induced ocular hypertension in mice.Experimental eye research · 2013Review
- BDNF treatment and extended recovery from optic nerve trauma in the cat.Investigative ophthalmology & visual science · 2013Article
- Optimizing gene transfer to conventional outflow cells in living mouse eyes.Experimental eye research · 2013Article
- Existence of the canonical Wnt signaling pathway in the human trabecular meshwork.Investigative ophthalmology & visual science · 2012Article
- Mutant human myocilin induces strain specific differences in ocular hypertension and optic nerve damage in mice.Experimental eye research · 2012Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeGene transduction using adenoviral vectors is an important research tool. To assess and optimize this technique for glaucoma research, we characterized green fluorescent protein (GFP) expression in the mouse eye after intraocular injection of adenoviral vector encoding GFP (Ad5.CMV-GFP) and evaluated the effect of anti-CD40L antibody administration on GFP expression.
methodsMice were injected with Ad5.CMV-GFP intracamerally (IC) or intravitreally (IVT) with or without anti-CD40L antibody treatment. GFP expression was assessed by in vivo fluorescence intensity with a standardized grading scale. Location of expression was analyzed histologically by fluorescence microscopy.
resultsIntraocular injection of Ad5.CMV-GFP induced titer-dependent expression of GFP in the anterior segment. In vivo fluorescence was detectable but low after IC injection. After IVT injection, fluorescence in the eye peaked at days 4-7 with a fluorescence grade of 3.0 +/-0.0 (mean +/-SEM, n=6; injection with 1x10(8) pfu vector). After day 7, GFP expression declined significantly. Treatment with anti-CD40L antibody increased fluorescence intensity after IC injection, and prolonged GFP expression in the IVT group. At day 43, fluorescence grades of the IVT group were 2.8 +/-0.7 (with anti-CD40L) and 1.2 +/-0.6 (without antibody). Three-Way ANOVA confirmed that GFP expression was significantly higher in the anti-CD40L than the no antibody group (p=0.013), significantly higher in the IVT than the IC group (p=0.003), and significantly higher in the high viral titer (1x10(8) pfu) than the low titer (1x10(7) pfu) group (p=0.010). Fluorescence microscopic examination of cross-sections of eyes indicated GFP expression in the trabecular meshwork (TM), corneal endothelium, and sporadically iris, ciliary body and lens epithelium.
conclusionsIntraocular injection of Ad5.CMV-GFP induced GFP expression in the mouse anterior segment, including the TM. Expression was more prominent after IVT injection than IC injection. Anti-CD40L antibody treatment increased both intensity and duration of GFP expression. These findings provide important and practical means to improve duration and efficiency of adenovirus-mediated transgene expression in the eye.
Indexed as
Identifiers
18246028PMC2267727W2237675506What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.