ArticleGenetics2008
The Saccharomyces cerevisiae homolog of p24 is essential for maintaining the association of p150Glued with the dynactin complex.
Article in Genetics, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
15 citing papers in PubMed, 22 citations in OpenAlex.
- VezA/vezatin facilitates proper assembly of the dynactin complex in vivo.Cell reports · 2024Article
- VezA/vezatin facilitates proper assembly of the dynactin complex in vivo.bioRxiv : the preprint server for biology · 2024Article
- Host Chromatin Regulators Required for Aggregatibacter actinomycetemcomitans Cytolethal Distending Toxin Activity in Saccharomyces cerevisiae Model.Infection and immunity · 2021Article
- Cryo-EM reveals the complex architecture of dynactin's shoulder region and pointed end.The EMBO journal · 2021Article
- Nuclear movement in fungi.Seminars in cell & developmental biology · 2018Review
- Dynactin integrity depends upon direct binding of dynamitin to Arp1.Molecular biology of the cell · 2014Article
- Systematic dissection of dynein regulators in mitosis.The Journal of cell biology · 2013Article
- Evolution of the eukaryotic dynactin complex, the activator of cytoplasmic dynein.BMC evolutionary biology · 2012Article
- The dynactin complex maintains the integrity of metaphasic centrosomes to ensure transition to anaphase.The Journal of biological chemistry · 2011Article
- Caenorhabditis elegans ortholog of the p24/p22 subunit, DNC-3, is essential for the formation of the dynactin complex by bridging DNC-1/p150(Glued) and DNC-2/dynamitin.Genes to cells : devoted to molecular & cellular mechanisms · 2010Article
- Parallel genetic and proteomic screens identify Msps as a CLASP-Abl pathway interactor in Drosophila.Genetics · 2010Article
- Stu1 inversely regulates kinetochore capture and spindle stability.Genes & development · 2009Article
- Function of dynein in budding yeast: mitotic spindle positioning in a polarized cell.Cell motility and the cytoskeleton · 2009Review
- Dynein-driven mitotic spindle positioning restricted to anaphase by She1p inhibition of dynactin recruitment.Molecular biology of the cell · 2009Article
- Cdc14 inhibition by the spindle assembly checkpoint prevents unscheduled centrosome separation in budding yeast.Molecular biology of the cell · 2009Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
Stu1 is the Saccharomyces cerevisiae member of the CLASP family of microtubule plus-end tracking proteins and is essential for spindle formation. A genomewide screen for gene deletions that are lethal in combination with the temperature-sensitive stu1-5 allele identified ldb18Delta. ldb18Delta cells exhibit defects in spindle orientation similar to those caused by a block in the dynein pathway. Consistent with this observation, ldb18Delta is synthetic lethal with mutations affecting the Kar9 spindle orientation pathway, but not with those affecting the dynein pathway. We show that Ldb18 is a component of dynactin, a complex required for dynein activity in yeast and mammalian cells. Ldb18 shares modest sequence and structural homology with the mammalian dynactin component p24. It interacts with dynactin proteins in two-hybrid and co-immunoprecipitation assays, and comigrates with them as a 20 S complex during sucrose gradient sedimentation. In ldb18Delta cells, the interaction between Nip100 (p150(Glued)) and Jnm1 (dynamitin) is disrupted, while the interaction between Jnm1 and Arp1 is not affected. These results indicate that p24 is required for attachment of the p150(Glued) arm to dynamitin and the remainder of the dynactin complex. The genetic interaction of ldb18Delta with stu1-5 also supports the notion that dynein/dynactin helps to generate a spindle pole separating force.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.