ArticleApplied and environmental microbiology2008
Use of Drosophila S2 cells as a model for studying Ehrlichia chaffeensis infections.
Article in Applied and environmental microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
11 citing papers in PubMed.
- Naturally Occurring Variability in the Regulatory Region of theInternational journal of molecular sciences · 2026Article
- Generating and working with Drosophila cell cultures: Current challenges and opportunities.Wiley interdisciplinary reviews. Developmental biology · 2019Review
- Alcohol resistance in Drosophila is modulated by the Toll innate immune pathway.Genes, brain, and behavior · 2016Article
- Role of JAK-STAT signaling in maturation of phagosomes containing Staphylococcus aureus.Scientific reports · 2015Article
- Drosophila Schneider 2 (S2) cells: a novel tool for studying HSV-induced membrane fusion.Virology · 2013Article
- The Wnt signaling pathway is involved in the regulation of phagocytosis of virus in Drosophila.Scientific reports · 2013Article
- Ehrlichia chaffeensis replication sites in adult Drosophila melanogaster.International journal of medical microbiology : IJMM · 2013Article
- Identification of critical host mitochondrion-associated genes during Ehrlichia chaffeensis infections.Infection and immunity · 2012Article
- The Drosophila melanogaster host model.Journal of oral microbiology · 2012Article
- How the insect immune system interacts with an obligate symbiotic bacterium.Proceedings. Biological sciences · 2011Article
- Ehrlichia chaffeensis infections in Drosophila melanogaster.Infection and immunity · 2009Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Ehrlichia chaffeensis is an obligate intracellular bacterium and the causative agent of human monocytic ehrlichiosis. Although this pathogen grows in several mammalian cell lines, no general model for eukaryotic cellular requirements for bacteria replication has yet been proposed. We found that Drosophila S2 cells are permissive for the growth of E. chaffeensis. We saw morulae (aggregates of bacteria) by microscopy, detected the E. chaffeensis 16S rRNA gene by reverse transcriptase PCR, and used immunocytochemistry to detect E. chaffeensis in S2 and mammalian cells. Bacteria grown in S2 cells reinfected mammalian macrophages. S2 cells were made nonpermissive for E. chaffeensis through incubation with lipopolysaccharide. Our results demonstrate that S2 cells are an appropriate system for studying the pathogenesis of E. chaffeensis. The use of a Drosophila system has the potential to serve as a model system for studying Ehrlichia due to its completed genome, ease of genetic manipulation, and the availability of mutants.
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