ArticleApplied and environmental microbiology2008
Characterization and application of a glucose-repressible promoter in Francisella tularensis.
Article in Applied and environmental microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 28 citations in OpenAlex.
- Host-pathogen interactions involved in erythrocyte invasion byFrontiers in cellular and infection microbiology · 2025Article
- Identification of an N-terminal tag (580N) that improves the biosynthesis of fluorescent proteins in Francisella tularensis and other Gram-negative bacteria.Molecular and cellular probes · 2024Article
- Phenotypic and transcriptional characterization ofFrontiers in microbiology · 2024Article
- PdpC, a secreted effector protein of the type six secretion system, is required for erythrocyte invasion byFrontiers in cellular and infection microbiology · 2022Article
- OpiA, a Type Six Secretion System Substrate, Localizes to the Cell Pole and Plays a Role in Bacterial Growth and Viability inJournal of bacteriology · 2020Article
- A predictedVirulence · 2019Article
- Mobilizable Plasmids for Tunable Gene Expression inFrontiers in cellular and infection microbiology · 2018Article
- Characterization of a Francisella tularensis-Caenorhabditis elegans Pathosystem for the Evaluation of Therapeutic Compounds.Antimicrobial agents and chemotherapy · 2017Article
- Temperature-Dependent Gentamicin Resistance of Francisella tularensis is Mediated by Uptake Modulation.Frontiers in microbiology · 2016Article
- Genetic engineering of Francisella tularensis LVS for use as a novel live vaccine platform against Pseudomonas aeruginosa infections.Bioengineered · 2015Article
- Synthetic promoters functional in Francisella novicida and Escherichia coli.Applied and environmental microbiology · 2014Article
- The use of resazurin as a novel antimicrobial agent against Francisella tularensis.Frontiers in cellular and infection microbiology · 2013Article
- Article
- TetR-based gene regulation systems for Francisella tularensis.Applied and environmental microbiology · 2012Article
- A Francisella tularensis live vaccine strain that improves stimulation of antigen-presenting cells does not enhance vaccine efficacy.PloS one · 2012Article
- New vector tools with a hygromycin resistance marker for use with opportunistic pathogens.Molecular biotechnology · 2011Article
- Development of transcriptional fusions to assess Leptospira interrogans promoter activity.PloS one · 2011Article
- Identification and characterization of novel and potent transcription promoters of Francisella tularensis.Applied and environmental microbiology · 2011Article
- Francisella tularensis DeltapyrF mutants show that replication in nonmacrophages is sufficient for pathogenesis in vivo.Infection and immunity · 2010Article
- Utilization of an unstable plasmid and the I-SceI endonuclease to generate routine markerless deletion mutants in Francisella tularensis.Journal of microbiological methods · 2010Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Francisella tularensis, the causative agent of tularemia, is a category A biodefense agent. The examination of gene function in this organism is limited due to the lack of available controllable promoters. Here, we identify a promoter element of F. tularensis LVS that is repressed by glucose (termed the Francisella glucose-repressible promoter, or FGRp), allowing the management of downstream gene expression. In bacteria cultured in medium lacking glucose, this promoter induced the expression of a red fluorescent protein allele, tdtomato. FGRp activity was used to produce antisense RNA of iglC, an important virulence factor, which severely reduced IglC protein levels. Cultivation in glucose-containing medium restored IglC levels, indicating the usefulness of this promoter for controlling both exogenous and chromosomal gene expression. Moreover, FGRp was shown to be active during the infection of human macrophages by using the fluorescence reporter. In this environment, the FGRp-mediated expression of antisense iglC by F. tularensis LVS resulted in reduced bacterial fitness, demonstrating the applicability of this promoter. An analysis of the genomic sequence indicated that this promoter region controls a gene, FTL_0580, encoding a hypothetical protein. A deletion analysis determined the critical sites essential for FGRp activity to be located within a 44-bp region. This is the first report of a conditional promoter and the use of antisense constructs in F. tularensis, valuable genetic tools for studying gene function both in vitro and in vivo.
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