ArticleExperimental hematology2008
Combinatorial regulation of novel erythroid gene expression in zebrafish.
Article in Experimental hematology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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20 citing papers in PubMed, 29 citations in OpenAlex.
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- NCOA4 is regulated by HIF and mediates mobilization of murine hepatic iron stores after blood loss.Blood · 2020Article
- Ppargc1a Controls Ciliated Cell Development by Regulating Prostaglandin Biosynthesis.Cell reports · 2020Article
- Tfap2a is a novel gatekeeper of nephron differentiation during kidney development.Development (Cambridge, England) · 2019Article
- Prostaglandin signaling regulates renal multiciliated cell specification and maturation.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Iroquois transcription factor irx2a is required for multiciliated and transporter cell fate decisions during zebrafish pronephros development.Scientific reports · 2019Article
- Homeogene emx1 is required for nephron distal segment development in zebrafish.Scientific reports · 2018Article
- Article
- The zebrafish kidney mutant zeppelin reveals that brca2/fancd1 is essential for pronephros development.Developmental biology · 2017Article
- PCBP1 and NCOA4 regulate erythroid iron storage and heme biosynthesis.The Journal of clinical investigation · 2017Article
- Advances in understanding the mechanisms of erythropoiesis in homeostasis and disease.British journal of haematology · 2016Review
- Nephrotoxin Microinjection in Zebrafish to Model Acute Kidney Injury.Journal of visualized experiments : JoVE · 2016Article
- Epithelial cell fate in the nephron tubule is mediated by the ETS transcription factors etv5a and etv4 during zebrafish kidney development.Developmental biology · 2016Article
- Nephron proximal tubule patterning and corpuscles of Stannius formation are regulated by the sim1a transcription factor and retinoic acid in zebrafish.Developmental biology · 2015Article
- Zebrafish pronephros tubulogenesis and epithelial identity maintenance are reliant on the polarity proteins Prkc iota and zeta.Developmental biology · 2014Article
- Temporal and spatial expression of tight junction genes during zebrafish pronephros development.Gene expression patterns : GEP · 2014Article
- Five transcription factors and FGF pathway inhibition efficiently induce erythroid differentiation in the epiblast.Stem cell reports · 2014Article
- A Cdx4-Sall4 regulatory module controls the transition from mesoderm formation to embryonic hematopoiesis.Stem cell reports · 2013Article
- Glcci1 deficiency leads to proteinuria.Journal of the American Society of Nephrology : JASN · 2011Article
Corrections and comments
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Authors and funding
5 authors at 5 institutions in 2 countries.
Funding
Abstract
objectiveThe specification and differentiation of hematopoietic stem cells into red blood cells requires precise coordination by multiple transcription factors. Most genes important for erythroid maturation are regulated by the Gata family of DNA-binding proteins. Previously, we identified three novel genes kelch-repeat containing protein (krcp), kiaa0650, and testhymin/glucocorticoid inducible transcript 1 (glcci1) to be expressed in erythroid cells in a Gata-independent manner, and we sought to further understand how these transcripts are regulated during zebrafish hematopoiesis. MATERIALS AND
methodsWe employed a loss-of-function approach, using combinations of antisense morpholinos to hematopoietic transcription factors and assayed for changes in gene expression in zebrafish embryos.
resultsUpon examination of embryos deficient for Gata1, Gata2, Biklf, and/or Scl, we found distinct gene combinations were required for expression of the novel genes. While krcp expression was dependent upon Gata1 and Biklf, kiaa0650 expression was greatly reduced and glcci1 was maintained in Gata1/Gata2/Biklf-deficient embryos. As with the gata1 gene, kiaa0650 and krcp required Scl for blood expression. Although reduced, glcci1 was expressed in posterior blood precursors in the absence of Scl and Gata2.
conclusionsThis work identifies glcci1 as having Scl-independent expression in the posterior hematopoietic mesoderm, suggesting that its posterior expression is activated by factors upstream or parallel to Scl and Gata2. Additionally, these studies establish that blood gene expression programs are regulated by transcription factors acting in combination during erythroid maturation.
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